FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n02_04b9251010.3520000005a58a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n02_04b9251010.3520000005a58a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48453
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAC150.0049523762163.333336
GCCATAG150.0049523762163.333332
TCCTGGA550.002660108366.8181842
TACAGGA600.0037552961.255
ATTTAGA852.0521124E-457.6470576
CTTTGAG902.721773E-454.4444477
CACTGTT750.00907933949.0000044
AGCTGTT750.00907933949.0000047
AGAGACG750.00907933949.0000048
TCCGTCG150.0091230349.0235-239
CGTCGAG150.0091230349.0235-239
AATGGTC350.007199316328.000002120-124
CTCACGA652.3760171E-422.615385225-229
CCTATAC650.00630698418.846155235-239
ATCAACA1452.3494755E-413.5172415230-234
AACAACA4000.00271026056.7374997230-234
TTTTTTT11201.88993E-95.906250525-29
Produced by FastQC (version 0.11.2)