FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_vtc5b3.3510000005a372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_vtc5b3.3510000005a372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124837
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5430.4349671972251817No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4790.38370034525020624No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT4080.326826181340468No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3050.244318591443242No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2550.2042663633377925No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2510.2010621850893565No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT2380.19064860578193965No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2360.18904651665772168No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT2240.1794339819124138No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2230.1786329373503048No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT2040.163413090670234No Hit
GAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT2040.163413090670234No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2030.162612046108125No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2000.16020891242179802No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC1930.15460160048703508No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1930.15460160048703508No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1740.13938175380696427No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1670.13377444187220136No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT1640.13137130818587436No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG1620.1297692190616564No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1560.12496295168900247No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1540.12336086256478447No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1520.1217587734405665No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA1500.12015668431634853No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1450.11615146150580358No Hit
CATTAATACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC1430.11454937238158558No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1430.11454937238158558No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1390.11134519413314964No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG1370.10974310500893164No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA1370.10974310500893164No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1360.10894206044682266No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1340.10733997132260469No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1330.10653892676049567No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1330.10653892676049567No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA1300.10413579307416872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG700.0175.09
CTACTTG150.00496169163.33333245
TACTGAT150.00496169163.33333245
CGAAAGC900.0149.722234
GGTCGTT251.1622595E-4147.07
GCAGGGT1750.0147.09
AGGTCGT251.1622595E-4147.06
CAAAAGC6100.0118.483614
GAGCAAA6250.0115.641
TCTATGC458.823023E-6108.888893
AAGCAGG7800.0108.365397
ACAAGGT354.4359427E-4105.03
GCAAAAG7800.092.660263
TCGTACT407.542917E-491.874992
AGCAGGG4300.091.1627968
AGCAGGT2900.088.70698
AGCAAAA8100.087.716052
TTTAAAC450.001204301381.666673
GCGAAAG1650.081.6666643
GCAGGTA2300.079.891319
Produced by FastQC (version 0.11.2)