FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_vtc2c7.3510000005a359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_vtc2c7.3510000005a359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138746
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT8780.6328110359938305No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5770.4158678448387701No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5550.4000115318639817No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5050.3639744569212806No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4970.35820852493044847No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT4230.3048736540152509No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC3620.2609084225851556No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3510.25298026609776136No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA2990.21550170815735226No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA2920.21045651766537413No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2800.2018076196791259No Hit
GATTTAGAAGGTTCCATTGGGAAAGTCTGTAGGACTCTATTGGCTAAGTC2670.1924379801940236No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA2640.19027575569746155No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT2540.18306834070892133No Hit
GCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC2360.17009499372954898No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2180.1571216467501766No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC2100.15135571475934442No Hit
GAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT2080.14991423176163637No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT2080.14991423176163637No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2060.14847274876392832No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT1940.1398238507776801No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1930.13910310927882608No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT1780.12829198679601575No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1720.1239675378028916No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1620.1167601228143514No Hit
AGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1600.11531863981664336No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1600.11531863981664336No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1580.11387715681893532No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC1570.1131564153200813No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG1560.11243567382122728No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1530.11027344932466522No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC1510.10883196632695717No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA1490.10739048332924914No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1480.10666974183039511No Hit
TTTTTAAACTATTCAACATTAATTGATGGCCATCCGAATTCTTTTGGTCG1470.10594900033154109No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1450.10450751733383305No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1430.10306603433612502No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1410.10162455133841697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATTG100.0014748066245.000022
GCAGGGG1000.0196.09
AGGGTAT150.0049622827163.333336
CAGTACG750.0147.09
CGAAAGC1600.0122.500014
GAGCAAA9350.0121.844921
CAAAAGC9650.0116.787574
AGCAGGT5600.0115.93758
AAGCAGG11550.0114.5454567
GCAGGTA3800.0112.828949
AGGGTGT354.436996E-4105.06
GCAGGGT2200.0100.227279
GCAAAAG11500.098.03
CAGGGTG1150.095.869568
AGCAAAA11950.095.334732
AGCAGGG4500.092.5555658
TTTAAAC407.5447035E-491.875013
AAGGTAC407.5447035E-491.875019
AGGGTGA1200.091.8759
AGCGAAA2300.085.217392
Produced by FastQC (version 0.11.2)