FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_vtc2c11.3510000005a365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_vtc2c11.3510000005a365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150396
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT7750.5153062581451634No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7510.49934838692518413No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5480.36437139285619297No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT4870.32381180350541233No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4060.26995398813798244No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3570.2373733343971914No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC3460.23005931008803424No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3310.22008564057554725No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3110.20678741455889782No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA2850.18949972073725366No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT2590.17221202691560944No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2550.1695523817122796No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2470.16423309130561983No Hit
GGTATCTTTGAGAACTCATGCCTTGAAACAATGGAAGTCGTTCAACAAAC2310.15359451049230033No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT2280.15159977658980292No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2220.1476103087848081No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT2150.14295592967898083No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA2040.13564190536982365No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC2030.13497699406899122No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC1940.12899279236149896No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1920.12766296975983404No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC1880.12500332455650415No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1840.12234367935317428No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1780.11835421154817947No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1780.11835421154817947No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1770.117689300247347No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG1760.11702438894651453No Hit
GCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC1730.11502965504401712No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG1650.10971036463735737No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1640.10904545333652492No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1640.10904545333652492No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA1630.10838054203569243No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA1590.10572089683236258No Hit
GAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAATGCT1580.1050559855315301No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1550.1030612516290327No Hit
CATTAATACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC1540.1023963403282002No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1530.10173142902736776No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1520.10106651772653528No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1520.10106651772653528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGTT100.0014749294245.000021
TTTAAAC301.174556E-6163.333333
GCAGGGG1050.0151.666669
GCAGGGT2150.0148.139539
ACAAGGT352.5300778E-6139.999983
GCAGGTA3850.0136.818189
CGAAAGC1600.0122.500014
GAGCAAA9700.0116.185571
CAAAAGC9450.0112.777794
GCAAAAG9900.0110.126263
AAACTAT458.828947E-6108.888896
AGGGTGT458.828947E-6108.888896
CAGTACG901.8189894E-12108.888899
AGCAGGG4550.0107.692318
AGCAGGT5900.0105.889838
AAGCAGG12000.0105.1458367
CAACTCT654.829253E-794.230774
AGCGAAA2100.093.333332
AGCAAAA12000.092.8958362
TAAACTA552.3917359E-589.090915
Produced by FastQC (version 0.11.2)