FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_vtc10d1.3510000005a38f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_vtc10d1.3510000005a38f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170097
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA12460.7325232073463965No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG7850.4615013786251374No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT6420.3774317007354627No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT5430.31922961604261096No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT4810.28277982562890586No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3770.22163824170914243No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3610.21223184418302496No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA3370.19812224789384883No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT3180.18695215083158434No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3070.18048525253237857No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2820.16578775639782006No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA2630.1546176593355556No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG2600.15285395979940858No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2440.1434475622732911No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2420.14227176258252644No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2340.13756856381946772No Hit
CCCTAATACAGTCTCTAGTTTCCAAGATATACTGCTGAGGATGTCAAAAA2310.1358048642833207No Hit
GTATTAGGGTCCATGGGCCCAGGGTTTTCCTCCACGTCTCCAGCCTGCTT2280.13404116474717367No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG2270.13345326490179132No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC2270.13345326490179132No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA2240.1316895653656443No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG2210.1299258658294973No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2170.12757426644796793No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT2160.12698636660258558No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA2050.12051946830337983No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT2040.1199315684579975No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG1990.11699206923108581No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA1920.11287677031340941No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1900.11170097062264472No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT1890.11111307077726239No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1880.11052517093188005No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1860.10934937124111535No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA1860.10934937124111535No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1830.10758567170496833No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA1830.10758567170496833No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA1800.1058219721688213No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG1800.1058219721688213No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA1730.10170667325114494No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1730.10170667325114494No Hit
GCATTATATGTCCAAATGTCCAGAAATCCATCATCAACTTTTTTATTTAA1710.10053087356038026No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1710.10053087356038026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1000.0245.01
GCAGGGG2650.0166.415089
ACCTAGA150.0049632634163.333331
CTCCTAC150.0049632634163.33333245
CAATAGT150.0049632634163.33333245
GCAAAAG7250.0157.137923
AGCAAAA8100.0140.648152
AAGGGTG352.531042E-6140.09
AGCGAAA1800.0136.111112
GCAGGGT2650.0124.811329
GTAAATA302.4037415E-4122.54
AGCAGGG6400.0120.585938
CAAAAGC9500.0118.6315844
CTATTCG354.4387404E-4105.09
CAAGGGT501.4906909E-598.08
AAGCAGG14200.096.619727
GAGCAAA12200.093.381141
GAGAGGC407.547664E-491.874999
AATAGTA407.547664E-491.874994
ATAGTAG407.547664E-491.874995
Produced by FastQC (version 0.11.2)