FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_rdentocariosa.3510000005a54a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_rdentocariosa.3510000005a54a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140782
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAGT655.4755023E-575.384616
ATAGGAG707.904506E-570.03
GTATAGG801.5306982E-461.2500041
TATAGGA902.7397307E-454.4444472
CCCTTAA953.5780092E-451.5789451
CCCTATA1159.1754296E-442.6086962
ATATCAC1601.0051106E-438.281253
TAGACCG1350.00201800436.2962955
TATGGTC1400.002412251435.05
CCATGCT1400.002412251435.07
GTGTAGG1400.002412251435.01
TATACGA1400.002412251435.02
TCTATGA1500.003382967632.6666688
GTAGACC1600.0046398230.6250024
TTTAACA2003.7288613E-430.6252
TTAATAG1650.005393257429.696972
ACTGTGC1650.005393257429.696978
TCTCATA1700.006240309728.8235282
TTAATAC1800.0082486927.2222233
GTCATAG2257.4290624E-427.2222211
Produced by FastQC (version 0.11.2)