FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_qiaamp_kit1.3510000005a5c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_qiaamp_kit1.3510000005a5c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT40.196174595389897No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG100.0013681364245.000027
GACCCGC100.0013681364245.000026
GCGAGGA150.004604407163.333337
Produced by FastQC (version 0.11.2)