FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_pacnes.3510000005a3b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_pacnes.3510000005a3b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107708
Sequences flagged as poor quality0
Sequence length251
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT450.001203850381.666671
GTACCTC750.009109369549.0000046
CCACCGT750.009109369549.0000046
TACTAGA1506.860966E-540.8333362
ACTAGAT1558.323695E-539.516133
GCTGGGT300.003391414432.66666825-29
TTCGTCT1550.003968080531.6129029
TATAGTC1700.00623389128.8235288
TCATAGA1750.007181843727.9999982
ATAGTTG1750.007181843727.9999983
TATATAG3501.3835197E-524.4999985
TATATTA6802.910383E-1123.4191175
TAACACT2700.002146675722.6851864
TATTATA6756.5847416E-1021.7777792
ATATACT2950.003588980520.7627124
TTACATC2950.003588980520.7627124
ATATATG3554.2938514E-420.7042261
TTATATT8905.0931703E-1119.2696634
ATTATAT8758.367351E-1018.23
GTATTAG4050.001042412418.1481481
Produced by FastQC (version 0.11.2)