FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_ny238plasmidsmix1rep.3510000005a34c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_ny238plasmidsmix1rep.3510000005a34c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92850
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2440.26278944534194937No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA2290.2466343564889607No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG2170.23371028540656974No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT1930.2078621432417878No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT1890.2035541195476575No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC1450.156165858912224No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT1350.14539579967689822No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG1270.1367797522886376No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1220.13139472267097468No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG1200.12924071082390953No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT1130.12170166935918149No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG1080.11631663974151858No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT1050.11308562197092084No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT1030.11093161012385568No Hit
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG1020.10985460420032309No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT990.10662358642972536No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT990.10662358642972536No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC980.1055465805061928No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG980.1055465805061928No Hit
TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT960.10339256865912762No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT204.7722322E-5183.750028
TAATAAG150.0049596555163.333334
CGCTGAC150.0049596555163.333337
AGAGTGG150.0049596555163.333335
TTTGGGT354.4323254E-4105.000018
TACAGGG354.4323254E-4105.000013
GGGCGCG407.5367815E-491.875017
CTAAACA450.001203323781.666679
TTGGGTA450.001203323781.666679
GTCCAGA707.890355E-570.000011
TATACTG707.890355E-570.000015
TACTGAT550.002667822466.8181847
ACTAAAC600.003766156561.258
AGGGGTG600.003766156561.258
AGGGCGC600.003766156561.256
GGGGTGA700.00693202752.5000049
TGGAGGG750.00910543949.05
CCTAGAG150.0091282649.025-29
CGCCTCA150.0091282649.045-49
ACAGGGC750.00910543949.04
Produced by FastQC (version 0.11.2)