FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_ny238cvp.3510000005a3a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_ny238cvp.3510000005a3a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132483
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG6450.4868549172346641No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6140.46345568865439346No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT4140.31249292362038905No Hit
ACATAATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGTTC3650.27550704618705796No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3420.2581463282081474No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC3350.2528626314319573No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3140.23701154110338682No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT3010.22719896137617657No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT2640.19927084984488574No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2500.18870345629250546No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT2480.1871938286421654No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2390.18040050421563522No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT2340.1766264350897851No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2310.17436199361427504No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT2140.16153015858638467No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2040.15398202033468444No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC1930.1456790682578142No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1860.14039537148162406No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1770.13360204705509385No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1760.13284723322992384No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1750.13209241940475383No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1720.12982797792924375No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA1720.12982797792924375No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG1690.1275635364537337No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1640.12378946732788357No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA1640.12378946732788357No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1630.12303465350271356No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1610.12152502585237351No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG1600.12077021202720349No Hit
ATTATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC1590.12001539820203347No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1550.11699614290135339No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1540.11624132907618336No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1520.11473170142584331No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1450.10944800464965315No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA1440.10869319082448313No Hit
CCTACCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCT1380.10416430787346301No Hit
GTACATAATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT1370.103409494048293No Hit
ACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGTTC1360.10265468022312298No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA1350.10189986639795295No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC1330.1003902387476129No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCC100.0014747318244.999982
GCCGAGG100.0014747318244.999986
CTAGCCG100.0014747318244.999983
GCAGGGT1850.0165.540549
TTTAAAC452.8194336E-10163.333343
GTACTTT150.0049620313163.333336
AGGGTGT402.6671842E-8153.124986
TAAACTA505.875336E-10146.999985
GGTAGTT251.16241965E-4146.999982
CATAATA1050.0140.02
CGAAAGC1200.0122.54
TAGTTTT302.4025523E-4122.54
GCAGGGG757.4578566E-11114.3333369
CAAAAGC6650.0105.000014
GAGCAAA6650.0103.15791
TAATAGG1450.0101.379314
GCTTATA501.4894675E-597.999992
GCAGGTA3150.093.333339
AGCAGGG4500.089.8333368
GCAAAAG7700.089.0909043
Produced by FastQC (version 0.11.2)