FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_ltcontamination.3510000005a557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_ltcontamination.3510000005a557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147624
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGGT550.002670949366.8181848
TTTAGGC750.00911601549.0000042
GTTTAGG750.00911601549.0000041
GTGTATA1558.3387284E-539.516131
CCAGACC1450.002865630433.7931024
GTATGTG1550.0039728131.6129021
GTACAGG2403.014737E-530.6251
CTATAAT1600.00464054630.6249982
TATTAAG1800.0082499727.2222232
ATAAGAG1800.0082499727.2222235
TACAGGA3151.9178138E-423.3333342
CTCCATA2950.003595206420.7627121
ATATTTA3200.005756310719.1406231
TGAACAG3500.00965159817.5000025
ATGAACA4550.00227811216.1538474
TGATAGG1450.003615014311.82758610-14
Produced by FastQC (version 0.11.2)