FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_gv1291.3510000005a1b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_gv1291.3510000005a1b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139642
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA36292.5987883301585484No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA24261.737299666289512No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT16031.1479354348978101No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA15701.1243035762879363No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC15151.0849171452714799No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT11350.8127927127941451No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC10610.759800060153822No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC9240.661692041076467No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT7980.5714613082024033No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA7000.5012818493003538No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT6870.4919723292419186No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT6370.45616648286332195No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC6240.44685696280488674No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG5440.3895676085991321No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA5360.3838386731785566No Hit
CATTTAGGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTG5110.36593574998925826No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA4990.35734234685839505No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG4820.34516835908967214No Hit
ATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAG4630.33156213746580543No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT4220.30220134343535615No Hit
ATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGA4140.29647240801478064No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA4120.2950401741596368No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4030.2885951218114894No Hit
GTATCAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3930.28143395253577No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3720.2663954970567594No Hit
TGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAAC3710.2656793801291875No Hit
GTATTAATTGATGGCCATCCGAATCCTTTTGGTCGCTGTCTGGCTGTCAG3570.2556537431431804No Hit
GACTTGAAGATGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG3510.2513570415777488No Hit
TTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT3460.24777645693988912No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG3380.2420475215193137No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT3190.22844129989544695No Hit
ATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTT3180.22772518296787497No Hit
AATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA3180.22772518296787497No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC3170.22700906604030308No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT2990.21411896134400826No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA2980.21340284441643634No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG2940.21053837670614856No Hit
GAAATAGCTCTCAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCT2870.20552555821314505No Hit
GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTG2820.20194497357528535No Hit
GAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTA2760.19764827200985374No Hit
CTAAAGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAGTGAGCGA2700.19335157044442217No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCA2690.19263545351685024No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC2680.1919193365892783No Hit
CAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGAT2680.1919193365892783No Hit
CGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCG2660.19048710273413444No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2640.18905486887899056No Hit
ATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT2580.18475816731355896No Hit
ATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAA2550.18260981653084316No Hit
GATTCGCTTGGAGAAGCTGTGATGAGAATGGGAGACCTTCATTACCTCCA2550.18260981653084316No Hit
AGTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCT2510.17974534882055543No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2480.17759699803783963No Hit
GAATCCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATACTAGAGTCCCGT2480.17759699803783963No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC2470.17688088111026767No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT2450.17544864725512382No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2440.17473253032755187No Hit
TTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTG2430.17401641339997995No Hit
ATTATATTCAGTATGGAAAGAGTAAAAGAACTACGGAATCTAATGTCGCA2380.1704358287621203No Hit
AAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATG2370.16971971183454834No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2330.16685524412426062No Hit
GTATGGAAAGAGTAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2320.1661391271966887No Hit
GCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGAC2320.1661391271966887No Hit
CTTGAATGGAATGGTAACACAGTTCGAGTCTCTGAAAATATACAGAGATT2260.1618424256312571No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT2260.1618424256312571No Hit
GGCCCACATGATGAACCCCAACAAAGCAACACAAAGCAAAAAACATGATA2250.16112630870368513No Hit
TACAGCCCCCATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCAC2250.16112630870368513No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2230.15969407484854128No Hit
GTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATT2220.15897795792096933No Hit
TCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT2200.15754572406582548No Hit
ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGG2190.15682960713825353No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT2190.15682960713825353No Hit
ATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCC2180.1561134902106816No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2150.1539651394279658No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2150.1539651394279658No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2130.15253290557282193No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2110.15110067171767808No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATCCTTTTGGTCG2090.1496684378625342No Hit
AATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGA2070.14823620400739032No Hit
CACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGC2070.14823620400739032No Hit
ATCCCCTTAGTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCAT2060.1475200870798184No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT2060.1475200870798184No Hit
GTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCTC2030.1453717362971026No Hit
CTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTT2000.1432233855143868No Hit
GCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC1970.141075034731671No Hit
GCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTG1960.14035891780409906No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT1950.1396428008765271No Hit
ATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAACACA1950.1396428008765271No Hit
ATTCAGTATTAATTGATGGCCATCCGAATCCTTTTGGTCGCTGTCTGGCT1950.1396428008765271No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGAAAAG1930.13821056702138326No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC1910.13677833316623939No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1880.13462998238352358No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTAATTAATGCA1880.13462998238352358No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG1870.13391386545595166No Hit
GAAAAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAA1870.13391386545595166No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1850.13248163160080778No Hit
ATTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT1850.13248163160080778No Hit
GAAGATACGGACCAGCATTAAGCATCAATGAACTGAGTAACCTTGCAAAA1840.13176551467323586No Hit
GTCACTGTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGC1840.13176551467323586No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1840.13176551467323586No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC1830.1310493977456639No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCACT1820.13033328081809198No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1800.1289010469629481No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC1790.12818493003537618No Hit
GGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCC1770.1267526961802323No Hit
CCTAACGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA1720.12317211154237265No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1710.1224559946148007No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1700.12173987768722878No Hit
ACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCTGCTGT1690.12102376075965685No Hit
CTCCAACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCT1680.12030764383208489No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA1670.11959152690451298No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1650.11815929304936909No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1650.11815929304936909No Hit
ATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAA1640.11744317612179718No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC1640.11744317612179718No Hit
TGGTAACACAGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA1580.11314647455636558No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA1570.11243035762879364No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1560.11171424070122168No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT1560.11171424070122168No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1560.11171424070122168No Hit
AGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTG1560.11171424070122168No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG1540.11028200684607783No Hit
GGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGT1540.11028200684607783No Hit
GCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCAC1530.10956588991850588No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA1510.10813365606336203No Hit
ATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTT1500.10741753913579008No Hit
CCTCATTATAGGTAAAGAAGACAGAAGATACGGACCAGCATTAAGCATCA1500.10741753913579008No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATT1490.10670142220821817No Hit
TTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTG1470.10526918835307428No Hit
ATCCCCTTAGTCAAAGGTGACAAGATTGGTCTTGTCTTTAGCCATTCCAT1470.10526918835307428No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1460.10455307142550237No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1450.10383695449793043No Hit
GTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATTGGGCAAGGAGAC1440.10312083757035848No Hit
CCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGC1440.10312083757035848No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1430.10240472064278656No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCATCTTA1400.10025636986007076No Hit
TATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTAT1400.10025636986007076No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1400.10025636986007076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT100.0014748168245.000026
CTACAGG100.0014748168245.00002245
GAGCGAA1600.0229.687521
GCAGGTC850.0172.941189
AGGAGAG150.004962317163.33333245
CACCGTC150.004962317163.333337
AGCAGGT2250.0136.111118
AAGCAGG11700.0135.06417
GAGCAAA8950.0134.134081
GCAGGGT9700.0128.814449
AGCAGGG9900.0128.686878
CAAAAGC9650.0125.673584
CAGTACG800.0122.500019
AGCGAAA3600.0119.097231
CGAAAGC3700.0115.878383
GCGAAAG3700.0115.878382
ATTAGTA1200.0112.2916641
TAAAGAT1000.0110.255
GACAGTA901.8189894E-12108.888897
TTAGTAG1250.0107.7999952
Produced by FastQC (version 0.11.2)