FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_gv1208.3510000005a1ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_gv1208.3510000005a1ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190188
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA59113.1079773697604476No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA37721.983300734010558No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA24711.2992407512566513No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC23681.2450838118072645No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT21841.1483374345384567No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT19621.0316108271815256No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC15960.8391696637011798No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC12630.6640797526657833No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT12070.6346352030622332No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA12030.6325320209476939No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT10110.5315792794498075No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG8730.4590194964982018No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA8370.4400908574673481No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT8260.434307106652365No Hit
CATTTAGGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTG8030.422213809493764No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA7970.41905903632195507No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC7600.3996046017624666No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7400.38908869118977013No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT7340.3859339180179612No Hit
ATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGA7300.38383073590342187No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT7260.38172755378888257No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA7140.3754180074452647No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG7040.37016005215891645No Hit
GTTTCTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT7000.36805687004437715No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG6900.3627989147580289No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA6670.3507056175994279No Hit
ATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAG6370.3349317517403832No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC6320.33230277409720904No Hit
TGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAAC5990.31495152165225987No Hit
TTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT5650.29707447367867584No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA5400.2839295854628052No Hit
ATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTT5350.28130060781963107No Hit
AATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA5220.2744652659473784No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG5180.2723620838328391No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT5040.26500094643195155No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG4690.2465981029297327No Hit
GAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTA4490.2360821923570362No Hit
CCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGC4370.2297726460134183No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGCTCCTG4370.2297726460134183No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG4310.22661787284160936No Hit
AGTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCT4310.22661787284160936No Hit
GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTG4210.22135991755526113No Hit
GTCAAAGGTGACAGAATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCTC4170.21925673544072183No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG4160.21873093991208697No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC4140.21767934885481735No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4020.21136980251119944No Hit
GACTTGAAGATGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG3990.20979241592529496No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG3990.20979241592529496No Hit
ATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAA3870.20348286958167708No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT3760.19769911876669402No Hit
CAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGAT3760.19769911876669402No Hit
TACAGCCCCCATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCAC3560.1871832081939975No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC3450.18139945737901444No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3450.18139945737901444No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC3440.18087366185037962No Hit
GCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGAC3430.1803478663217448No Hit
CGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCG3420.17982207079310997No Hit
AAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATG3400.17877047973584032No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT3360.17666729762130104No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA3330.17508991103539656No Hit
GATTACTGATCGTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACG3330.17508991103539656No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG3330.17508991103539656No Hit
TCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT3280.17246093339222243No Hit
TCTTAAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTC3210.16878036469177865No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3210.16878036469177865No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTAATTAATGCA3180.16720297810587417No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3180.16720297810587417No Hit
ATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAA3130.16457400046270007No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT3120.16404820493406524No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG3080.16194502281952594No Hit
CACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGC3080.16194502281952594No Hit
GCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC3070.16141922729089112No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA3070.16141922729089112No Hit
ATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCC3050.16036763623362146No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT3010.1582644541190822No Hit
GGCCCACATGATGAACCCCAACAAAGCAACACAAAGCAAAAAACATGATA3000.15773865859044733No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC3000.15773865859044733No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG2990.15721286306181254No Hit
ATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT2960.15563547647590806No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT2960.15563547647590806No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2910.15300649883273393No Hit
GTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATT2860.1503775211895598No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2820.14827433907502052No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC2780.14617115696048122No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG2770.1456453614318464No Hit
GAAATAGCTCTCAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCT2760.14511956590321154No Hit
TATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTAT2760.14511956590321154No Hit
GAAAAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAA2760.14511956590321154No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC2670.14038740614549813No Hit
GGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGCGTCTACGCTGCAGT2670.14038740614549813No Hit
ACATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTC2650.13933581508822848No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC2620.137758428502324No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2610.1372326329736892No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT2600.13670683744505435No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2590.13618104191641955No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC2550.13407785980188025No Hit
ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGG2540.13355206427324542No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGC2520.13250047321597577No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG2510.13197467768734095No Hit
CTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTT2510.13197467768734095No Hit
AATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGA2500.13144888215870612No Hit
GTCACTGTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGC2490.1309230866300713No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG2480.13039729110143647No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCA2480.13039729110143647No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT2480.13039729110143647No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC2470.12987149557280164No Hit
ATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAACACA2470.12987149557280164No Hit
GTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAG2450.12881990451553202No Hit
ATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTT2390.12566513134372306No Hit
CCTCTCGTCATTGCAGCAAACATCATTGGGATCTTGCACCTGATATTGTG2390.12566513134372306No Hit
TTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTG2350.12356194922918376No Hit
ATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAG2320.12198456264327928No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2310.12145876711464447No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT2280.11988138052873999No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2260.11882978947147034No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGC2260.11882978947147034No Hit
CATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCA2250.11830399394283551No Hit
GATTCGCTTGGAGAAGCTGTGATGAGAATGGGAGACCTTCACTACCTCCA2250.11830399394283551No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG2250.11830399394283551No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATAGGTC2240.1177781984142007No Hit
GTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCA2210.11620081182829621No Hit
GCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTG2210.11620081182829621No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2180.11462342524239173No Hit
GCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCAC2150.11304603865648725No Hit
GACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG2140.11252024312785244No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG2140.11252024312785244No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT2110.11094285654194797No Hit
ACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCA2100.11041706101331314No Hit
CATGATGAACCCCAACAAAGCAACACAAAGCAAAAAACATGATATGGCAA2070.10883967442740868No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG2070.10883967442740868No Hit
GGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCC2060.10831387889877384No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC2030.10673649231286936No Hit
GAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAG2030.10673649231286936No Hit
TAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGC2000.10515910572696491No Hit
AGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTG2000.10515910572696491No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2000.10515910572696491No Hit
TATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGT1990.10463331019833007No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1970.10358171914106043No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC1970.10358171914106043No Hit
CTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCA1950.10253012808379078No Hit
CTTGAGTGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATT1940.10200433255515594No Hit
GGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT1940.10200433255515594No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC1940.10200433255515594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3050.0236.967221
GCAGGGG304.78758E-9204.166679
TTTCTAA1300.0169.615392
TTCTAAT1350.0163.333343
CGTCCAA150.0049637216163.33333245
GAGCAAA12200.0148.606571
AGCAGGG14300.0139.632868
AAGCAGG18000.0139.513897
TCTAATT1600.0137.81254
GCAGGGT14250.0135.824559
CTAATTA1650.0133.636375
ATTAGTA1750.0126.000011
GCAGGTC1350.0117.962979
GCGAAAG6200.0116.572583
AGCGAAA6200.0116.572582
CGAAAGC6250.0115.644
AGCAGGT3350.0113.358218
GTAGAAA7750.0109.0645141
TTTTAAA1750.0105.000012
GCAGGTA2150.0102.558149
Produced by FastQC (version 0.11.2)