FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_a39rep.3510000005a563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_a39rep.3510000005a563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130531
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8350.6396947851468233No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5130.3930100895572699No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT5110.391477886479074No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4180.3202304433429607No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4130.3163999356474707No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2910.22293554787751568No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2220.17007454167975422No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2200.16854233860155826No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2180.16701013552336227No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2090.16011522167148035No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1770.1355999724203446No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1760.1348338708812466No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1760.1348338708812466No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1740.1333016678030506No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1710.13100336318575664No Hit
CGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATG1630.12487455087297271No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1600.12257624625567871No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1590.12181014471658073No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1560.11951184009928675No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1540.11797963702109078No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1540.11797963702109078No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1500.1149152308646988No Hit
GTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGATCGCATGACAC1420.10878641855191487No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1410.10802031701281688No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1380.10572201239552291No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT1380.10572201239552291No Hit
GCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGA1370.10495591085642492No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1350.10342370777822892No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1320.10112540316093495No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1320.10112540316093495No Hit
TTCTAATAGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCATG1310.10035930162183697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT1150.0149.130459
GCAGGGG1250.0147.09
AGCAGGT4700.0114.6808558
CAAAAGC7550.0111.953644
GCAAAAG7700.0111.363633
AGCAAAA7600.0111.21712
CGAAAGC1800.0108.888894
AAGCAGG9750.0106.7948767
GCTACTA654.8256516E-794.23077245
AGGGTGA403.743628E-491.874999
CAGGGTG407.543696E-491.874998
GAGCAAA9250.091.378371
GAGCGAA2200.089.090911
ACAAGGT552.390547E-589.090918
AAACTAT450.001204425481.666676
TTTAAAC450.001204425481.666673
GAATGTC1550.079.032267
AAAGCAG13850.075.1805046
AGCAGGG4750.074.7894748
AGCGAAA2700.072.592592
Produced by FastQC (version 0.11.2)