FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_80.3510000005a3f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_80.3510000005a3f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174890
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA5460.3121962376350849No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4030.23043055634970555No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3990.228143404425639No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3930.22471267653953914No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3810.21785122076733945No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA3320.18983360969752416No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG3240.18525930584939104No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3210.18354394190634113No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC3140.17954142603922466No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2910.16639030247584194No Hit
GCAAAAGCCCTTCTTTTTAATTTGCCTCTCTCTGCATCTTTGGTCATCGT2750.15724169477957572No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2740.15666990679855908No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2740.15666990679855908No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2640.15095202698839272No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2580.14752129910229286No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2560.1463777231402596No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2490.14237520727314312No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCA2420.13837269140602665No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2410.13780090342501No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2380.1360855394819601No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2330.13322659957687688No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2330.13322659957687688No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG2180.1246497798616273No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2160.12350620389959402No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC2100.1200754760134942No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2030.11607296014637773No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC2020.11550117216536109No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1960.11207044427926124No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1950.11149865629824461No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1920.1097832923551947No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC1920.1097832923551947No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1920.1097832923551947No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC1900.10863971639316142No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1860.10635256446909487No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1800.10292183658299503No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA1790.10235004860197838No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1750.10006289667791182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTGG100.0014751344245.000027
GGTGGCC100.0014751344245.000029
GTAACCG550.0222.727284
TAACCGT650.0188.461535
GTCCTAG150.004963383163.333331
TTGTAGG150.004963383163.333334
TGTAGGT150.004963383163.333335
AGGTGGC150.004963383163.333338
GCAGGGG458.833005E-6108.888889
TCCAACT1150.0106.5217362
GCAGGGT354.4389517E-4105.08
GTACTGA501.4908095E-598.01
TAACTAT1400.096.255
GCTATAA1450.092.931041
AAAAGCC801.5463229E-891.875013
GATCTTA407.5480214E-491.87501245
GTATTAA1200.091.8756
CTATAAC1500.089.8333362
TTGTCTC1101.6370905E-1189.090919
TACTGAT552.3928304E-589.090912
Produced by FastQC (version 0.11.2)