FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_76.3510000005a4de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_76.3510000005a4de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80895
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4720.5834724024970641No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT2560.31645960813400087No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAGTCATGG2180.26948513505161015No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1690.20891278818221148No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1550.19160640336238335No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1310.16193831509982076No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1260.15575746337845353No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1240.15328512268990666No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1130.13968724890289883No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1100.1359787378700785No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTTCCCGCGCTGGGGT1080.13350639718153162No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1070.13227022683725817No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1060.13103405649298475No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1040.12856171580443784No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1000.12361703442734409No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG970.11990852339452376No Hit
ACACTAAACAACGTGCATTCAAATAACACAGTACGTGATAGGACCCCTTA940.11620001236170344No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA920.11372767167315656No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA920.11372767167315656No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC880.1087829902960628No Hit
GTTCAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT870.10754681995178936No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA870.10754681995178936No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT860.1063106496075159No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG850.10507447926324247No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT840.10383830891896903No Hit
GTTTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT830.10260213857469559No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT820.10136596823042214No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC810.1001297978861487No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT810.1001297978861487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG251.6007107E-9244.999989
GCAGGGT500.0195.999989
CGAAAGC800.0168.43754
TGCGAGA302.3991233E-4122.5245
GCGAAAG1150.0117.173913
AAGCAGG3850.0105.000017
TCCAACT354.43023E-4105.000012
GAGCGAA1300.0103.6538541
AGCGAAA1350.099.814812
AGCAGGG1700.093.6764768
CCATGAA552.3850256E-589.090918
AGCAGGT1700.086.47068
CAAAAGC3000.085.750014
CATATTA751.1269658E-681.666675
TTATATT450.001202757281.666672
GTGCTCG450.001202757281.666679
GGGTTAA457.453328E-481.666671
TCAAGAG450.001202757281.666673
ATATTCA450.001202757281.666674
ACCATGA603.6725258E-581.6666647
Produced by FastQC (version 0.11.2)