000000000-AP76Y_l01n01_70.3510000005a273.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AP76Y_l01n01_70.3510000005a273.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	120333
Sequences flagged as poor quality	0
Sequence length	251
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA	930	0.7728553264690484	No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA	364	0.30249391272552	No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT	335	0.2783941229754099	No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT	297	0.24681508813043804	No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT	294	0.24432200643215077	No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG	261	0.216898107750991	No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC	254	0.21108091712165408	No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC	247	0.20526372649231714	No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC	236	0.19612242693193058	No Hit
TTTCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC	236	0.19612242693193058	No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT	232	0.1927983180008809	No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT	230	0.19113626353535607	No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG	227	0.1886431818370688	No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT	224	0.18615010013878155	No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG	222	0.18448804567325672	No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT	219	0.18199496397496945	No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA	205	0.17036058271629562	No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA	195	0.16205031038867146	No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG	192	0.1595572286903842	No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG	188	0.15623311975933452	No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC	183	0.1520779835955224	No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC	173	0.14376771126789825	No Hit
GTATAGAAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG	172	0.14293668403513582	No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA	172	0.14293668403513582	No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA	170	0.141274629569611	No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC	167	0.13878154787132374	No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG	164	0.1362884661730365	No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA	164	0.1362884661730365	No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA	157	0.13047127554369958	No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA	154	0.1279781938454123	No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG	151	0.12548511214712504	No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA	148	0.12299203044883782	No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT	148	0.12299203044883782	No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG	142	0.11800586705226329	No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGAAATGGTTTG	141	0.11717483981950089	No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT	138	0.11468175812121364	No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC	133	0.11052662195740154	No Hit
CTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACACT	133	0.11052662195740154	No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT	132	0.10969559472463913	No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA	131	0.1088645674918767	No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC	130	0.1080335402591143	No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC	127	0.10554045856082704	No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT	124	0.10304737686253977	No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC	124	0.10304737686253977	No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA	124	0.10304737686253977	No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT	123	0.10221634962977737	No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC	123	0.10221634962977737	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGGGT	120	0.0	163.33333	9
GGCATGA	15	0.004961469	163.33333	1
GACTACG	15	0.004961469	163.33333	245
TATATTC	60	2.0881998E-9	122.49999	3
CGAAAGC	140	0.0	113.75	4
AGCAAAA	555	0.0	112.567566	2
GCAGGGG	55	1.7826824E-7	111.36364	8
AAGCAGG	720	0.0	108.88888	7
GCAAAAG	590	0.0	105.889824	3
GCGAAAG	155	0.0	102.74193	3
GAGCGAA	160	0.0	99.53124	1
TTATATT	75	9.851647E-9	98.00001	2
TACGGAT	50	1.4889083E-5	98.0	2
CGGATAA	50	1.4889083E-5	98.0	4
ACGGATA	50	1.4889083E-5	98.0	3
CAAAAGC	700	0.0	92.74999	4
AGGGTGT	40	7.5422495E-4	91.87499	6
ATATTCA	80	1.5421392E-8	91.87499	4
GGTAGTT	55	2.3897841E-5	89.09091	7
ACAAGGT	70	7.497929E-7	87.5	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content