FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_69.3510000005a230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_69.3510000005a230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126500
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6530.516205533596838No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6260.4948616600790514No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6140.4853754940711463No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5540.4379446640316206No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA5300.41897233201581024No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4810.3802371541501976No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4650.3675889328063241No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA4490.3549407114624506No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA4490.3549407114624506No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC4440.3509881422924901No Hit
ACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCTGCTGT4260.3367588932806324No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4220.33359683794466405No Hit
CTCCAACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCT4100.3241106719367589No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3800.30039525691699603No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG3560.28142292490118576No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC3460.2735177865612648No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3220.2545454545454546No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3090.2442687747035573No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3080.2434782608695652No Hit
ATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2910.2300395256916996No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2840.22450592885375495No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2700.21343873517786563No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2580.20395256916996046No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2570.20316205533596837No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2330.1841897233201581No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT2300.18181818181818182No Hit
GTACTGGAACTAGGATGAGTCCCAATGGTTCTCATTGCCTGCACCATCTG2250.17786561264822134No Hit
CTCTTTGTCCTTGTTCATTTCTCTCCGGAACCATTTCTGTTATCCTTTTG2200.17391304347826086No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2160.1707509881422925No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2110.16679841897233202No Hit
TCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA2070.16363636363636364No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT2010.15889328063241107No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1960.1549407114624506No Hit
CTACTGTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCC1920.1517786561264822No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1900.15019762845849802No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG1810.14308300395256918No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1810.14308300395256918No Hit
TCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAA1780.14071146245059288No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1770.1399209486166008No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1770.1399209486166008No Hit
ACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGT1760.1391304347826087No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1750.1383399209486166No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1720.13596837944664034No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1720.13596837944664034No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1720.13596837944664034No Hit
TCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA1670.13201581027667983No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG1640.12964426877470356No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTGTTGTATA1610.1272727272727273No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1590.12569169960474308No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC1590.12569169960474308No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGCCGT1580.12490118577075097No Hit
CATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1580.12490118577075097No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA1570.1241106719367589No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1570.1241106719367589No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1560.1233201581027668No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1550.1225296442687747No Hit
GGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGG1530.12094861660079052No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC1530.12094861660079052No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1500.11857707509881424No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1490.11778656126482213No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT1480.11699604743083003No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1450.11462450592885376No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1440.11383399209486166No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT1430.11304347826086955No Hit
CAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1410.11146245059288537No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1370.108300395256917No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1370.108300395256917No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA1360.10750988142292489No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1360.10750988142292489No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC1350.10671936758893281No Hit
ATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAATCTCCAT1340.10592885375494071No Hit
CTCTATCTGATCCAGCATCACTCATTTTACTCCATAGAGTTTGTCCTTGT1340.10592885375494071No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGTTCTCATTGCC1340.10592885375494071No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1330.10513833992094862No Hit
GGCCTCCACCATGCTAGAAATTCCAATCGGTCTCCTATATGAACTACTAG1320.10434782608695652No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1320.10434782608695652No Hit
GAGAACCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAGATGATC1290.10197628458498023No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG1290.10197628458498023No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT1270.10039525691699605No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG1270.10039525691699605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG750.0212.333334
TAACCGT850.0187.352945
GTAGGCT150.0049617696163.333333
GCAGGGG150.0049617696163.333339
TGTAGGC150.0049617696163.333332
CGTACCA1150.0149.130459
TTTGTAG251.16229676E-4147.04
CCTGTAA1100.0144.772721
AACCGTA1200.0142.916676
CGAAAGC1550.0142.258073
GCGAAAG1500.0138.833332
GAGCGAA1050.0128.333331
GCAGGGT1150.0127.8260969
CAAAAGC6450.0125.348834
TACTATA302.4022997E-4122.52
CAGTACG1050.0116.6666649
ACCGTAC1550.0110.645177
AGCAGGG1650.0103.939398
CAACTCT1200.0102.0833364
AAGCAGG9200.0101.1956567
Produced by FastQC (version 0.11.2)