FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_56.3510000005a1fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_56.3510000005a1fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83727
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4620.5517933283170303No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3310.3953324495085218No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3290.39294373380152164No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2740.327254051859018No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2650.3165048311775174No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2230.26634180133051466No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2080.24842643352801363No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1900.22692799216501247No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1450.17318188875750953No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1380.16482138378300906No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1330.15884959451550873No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1190.14212858456650782No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1110.1325737217385073No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1100.1313793638850072No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1100.1313793638850072No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1100.1313793638850072No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1090.13018500603150715No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1020.12182450105700669No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1000.11943578535000655No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT980.11704706964300643No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG940.11226963822900617No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC940.11226963822900617No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC900.1074922068150059No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG870.10390913325450571No Hit
ATTTAAAGCAATATGATAGTGAAGAACCTGAATTAAGGTCACTTTCAAGC850.10152041754750557No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC850.10152041754750557No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT850.10152041754750557No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG150.004958787163.333337
GCAGGGG600.0163.333339
ATTGCGA150.004958787163.333338
GCAGGGT1250.0156.89
TTTAAAC251.1609017E-4147.03
TTTAAAG352.5234567E-6140.02
AAGCAGG5700.0122.500017
AGCAGGT2700.0122.58
GCAAAAG5200.0117.788473
CGAAAGC757.4578566E-11114.3333364
AGCAAAA5500.0111.363642
CAAAAGC5850.0104.700864
AGCAGGG2950.099.661028
GAGCAAA6200.098.790321
TTGCGAA407.5341633E-491.874999
TCGACAG552.3855191E-589.09091245
AGAGGAT603.6732847E-581.6666648
AAAGCAG8900.079.831466
AGCGAAA1101.5606929E-977.9545442
GAGCGAA955.0618837E-877.3684161
Produced by FastQC (version 0.11.2)