FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_32.3510000005a2c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_32.3510000005a2c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179496
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10930.608927218433837No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT6310.3515398671836698No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG5690.31699870749208897No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4520.2518161964612025No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3450.19220484021928066No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3210.17883406872576546No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3000.1671346436689397No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2970.16546329723225028No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2960.16490618175335384No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2930.16323483531666444No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2900.16156348887997504No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2900.16156348887997504No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2800.1559923340910104No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2790.15543521861211393No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2740.15264964121763158No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2730.15209252573873513No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2710.15097829478094218No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2570.14317867807639167No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2520.14039310068190936No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2470.13760752328742704No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2460.13705040780853056No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2420.1348219458929447No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2380.13259348397735882No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2330.1298079065828765No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT2300.1281365601461871No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2280.12702232918839418No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2210.12312252083611891No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG2150.11977982796274013No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2120.1181084815260507No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2060.11476578865267192No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2030.11309444221598254No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG2000.11142309577929313No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA1990.11086598030039665No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1970.10975174934260373No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA1910.10640905646922495No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1890.105294825511432No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1870.10418059455363908No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1850.10306636359584614No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT1850.10306636359584614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT100.001475167244.99998245
GAGCGAA700.0227.51
GCAGGGT1550.0165.967739
GTATTAA557.2759576E-12155.909091
TACGGAT700.0140.02
CGGATAA700.0140.04
GCGAAAG1200.0132.708333
ACGGATA750.0130.666673
CGAAAGC1150.0127.826084
AGCGAAA1250.0127.42
GCAGGGG1350.0117.962979
GCAAAAG7050.0114.6808553
GTCGTAC603.000314E-7102.0833361
ACAAGGT852.0372681E-10100.8823558
AAGCAGG9600.099.531257
AGCAGGT4800.094.4270868
CGTACTC654.833091E-794.230773
TCGTACT654.833091E-794.230772
CAAAAGC9100.091.538464
AGCAAAA8900.090.84272
Produced by FastQC (version 0.11.2)