FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_295.3510000005a0ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_295.3510000005a0ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159110
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT25721.616491735277481No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC12700.7981899314939349No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA11770.7397398026522531No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC10000.6284960090503425No Hit
GCAATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTT8610.5411350637923449No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC8410.528565143611338No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8070.5071962793036264No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA7900.49651184714977054No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA6860.43114826220853497No Hit
GCAATAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGA6720.42234931808183024No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA5960.37458362139400414No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5640.35447174910439316No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG5480.34441581295958773No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA5220.3280749167242788No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG4890.30733454842561747No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA4640.29162214819935894No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4620.29036515618125824No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT4540.2853371881088555No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4470.2809377160455031No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4130.25956885173779143No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3980.25014141160203635No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3900.2451134435296336No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG3780.23757149142102948No Hit
GTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGC3660.23002953931242537No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT3640.22877254729432467No Hit
AGTAGAAACAAGGTACTTTTTTTGGACAGTACGGATAACAAATAGTAGCA3630.22814405128527435No Hit
GACTTAGCCATTGATGAAATGGAAATCTGAATACATGAAACACACCTCCA3590.22563006724907295No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT3380.21243165105901576No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA3340.20991766702281442No Hit
ATATACAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGGT3290.2067751869775627No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3290.2067751869775627No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT3260.20488969895041168No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3210.20174721890515995No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT3140.19734774684180756No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3080.1935767707875055No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT3080.1935767707875055No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3060.19231977876940481No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3030.19043429074225376No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3010.1891772987241531No Hit
GTAGAAACAAGGTACTTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2910.18289233863364968No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2890.181635346615549No Hit
ATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCAT2850.1791213625793476No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCTAAGAG2750.17283640248884422No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2740.17220790647979387No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2690.16906542643454214No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAA2640.16592294638929042No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2600.16340896235308905No Hit
GATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAA2600.16340896235308905No Hit
CAATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTC2570.16152347432593803No Hit
GACTTATTGCTGCAAATTTGTTGGTTTCAATTTTCAGATCCTCCCCATAC2560.16089497831688768No Hit
AGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAA2560.16089497831688768No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2470.1552385142354346No Hit
GCAATGAAAGAGTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGC2470.1552385142354346No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2380.14958205015398152No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2320.14581107409967947No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG2280.1432970900634781No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC2270.14266859405442775No Hit
CCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAAC2260.14204009804537743No Hit
CAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2230.14015461001822638No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2230.14015461001822638No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC2210.1388976180001257No Hit
TTTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTA2190.137640625982025No Hit
TCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCA2160.13575513795487398No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2150.13512664194582363No Hit
TGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAAACCCAGGGATCAT2140.13449814593677328No Hit
CAATAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGAT2050.12884168185532022No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2040.1282131858462699No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2000.1256992018100685No Hit
GAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGT1960.12318521777386712No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC1900.11941424171956509No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT1880.11815724970146439No Hit
CTTAGGGACAAGCTCGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1880.11815724970146439No Hit
AAACAAGGTACTTTTTTTGGACAGTACGGATAACAAATAGTAGCACTGCT1850.11627176167431337No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1850.11627176167431337No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC1820.11438627364716233No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1790.11250078562001131No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTCA1780.11187228961096096No Hit
GCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAA1780.11187228961096096No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG1730.10872980956570925No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1720.10810131355665892No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGC1710.10747281754760858No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1690.10621582552950788No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1690.10621582552950788No Hit
GTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAAACCCAGGGATCATT1680.10558732952045756No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG1680.10558732952045756No Hit
CATATACAATAGGATGGGGGCTATAACCACTGAAGTGGCATTTGGCCTGG1640.10307334548425616No Hit
GCGAAAGAGTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGC1610.10118785745710515No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA1610.10118785745710515No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1610.10118785745710515No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1610.10118785745710515No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1600.1005593614480548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC100.00147501245.00002245
GCAGGGG700.0175.000029
GCAGGGT1900.0154.736839
GAGCGAA2300.0149.130431
AGCAGGG2900.0143.62078
AAGCAGG13100.0142.13747
CGCTCCC352.5305344E-6140.000026
GCTCCCC455.3933036E-8136.111117
CAAAAGC12150.0129.05354
AGCAGGT10400.0124.855768
CGAAAGC2900.0118.275864
GCAAAAG14300.0107.080423
AGGGTGT953.6379788E-12103.157896
GCAGGTA9050.0102.872939
AAAGCAG19200.097.617196
TCGCTCC654.830563E-794.230775
TTTAAAC1550.086.935493
AAAAGCA18050.086.191145
AAGGTAC1300.084.8076869
GCAGGTC4350.081.6666649
Produced by FastQC (version 0.11.2)