FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_291.3510000005a0c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_291.3510000005a0c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107371
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT12721.1846774268657272No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT11411.062670553501411No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8860.8251762580212534No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC8410.7832654999953432No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4190.3902357247301413No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4100.38185357312495927No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3890.3622952193795345No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3320.30920825921338163No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC3320.30920825921338163No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3290.306414208678321No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC3100.28871855528960333No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2890.2691602015441786No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2710.25239589833381454No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2710.25239589833381454No Hit
ATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2480.23097484423168269No Hit
CTCTTTGTCCTTGTTCATTTCTCTCCGGAACCATTTCTGTTATCCTTTTG2460.22911214387497553No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2440.22724944351826845No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2400.2235240428048542No Hit
CCCTTACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACACT2390.22259269262650067No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2320.21607324137802572No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2300.2142105410213186No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2260.21048514030790438No Hit
GGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGG2220.20675973959449015No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2200.20489703923778302No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1990.18533868549235827No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1970.18347598513565117No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1870.17416248335211554No Hit
CTTAAACTACACATACCCAGCATTGAACGTGACTATGCCAAACAATGAAC1870.17416248335211554No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC1840.17136843281705488No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1810.1685743822819942No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1810.1685743822819942No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1760.16391763139022641No Hit
TCCTCAAGAATTCCCCTTTGGCTTGTGTTTAGAATAGAGCGGTTCCTTTT1720.16019223067681218No Hit
GATCTTATGATCTCAGAGAACTCTTCCTTCTTAATCCTTCCAGACTCGAA1720.16019223067681218No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1710.1592608804984586No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTAATCCTTCCAGACTCGA1690.1573981801417515No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1610.14994737871492303No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1610.14994737871492303No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1600.14901602853656948No Hit
ACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCTGCTGT1600.14901602853656948No Hit
GTATTTTAAGTGGGTCAACCAATTTAATCTACTAAAGAAACTACTACTGG1600.14901602853656948No Hit
GTACTGGAACTAGGATGAGTCCCAATGGTTCTCATTGCCTGCACCATCTG1590.1480846783582159No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT1580.14715332817986235No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1560.14529062782315522No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1540.1434279274664481No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1540.1434279274664481No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1530.14249657728809456No Hit
CTCCAACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCT1520.14156522710974098No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1510.14063387693138743No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT1480.13783982639632675No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1470.1369084762179732No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1470.1369084762179732No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTTC1460.13597712603961964No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1390.12945767479114473No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1360.12666362425608405No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1300.1210755231859627No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT1300.1210755231859627No Hit
GAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGATGCAT1280.11921282282925556No Hit
GGGTACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGGGA1240.11548742211584134No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1240.11548742211584134No Hit
CTCTATCTGATCCAGCATCACTCATTTTACTCCATAGAGTTTGTCCTTGT1190.11083067122407354No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1180.10989932104571998No Hit
GTACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGGGAAG1140.10617392033230576No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTGTTGTATA1130.10524257015395219No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1110.10337986979724507No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1110.10337986979724507No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1110.10337986979724507No Hit
GTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGGGA1100.10244851961889151No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG1090.10151716944053794No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1090.10151716944053794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT1350.0163.333349
CAAAAGC9350.0161.149734
AGCAAAA10250.0145.804872
GCAAAAG10300.0145.097093
GCAGGGG850.0144.117669
AAGCAGG11900.0141.029427
GCAGGTA4800.0132.708339
AGCAGGG2750.0129.181828
GAGCAAA12000.0127.6041641
GCAGGTC2500.0127.3999949
AAAGCAG13600.0123.400756
GAGCGAA1600.0122.500011
AGCAGGA404.909627E-6122.500018
AGCAGGT7950.0120.1886758
GCGAAAG1700.0115.294133
AAAAGCA13800.0108.29715
CGAAAGC1850.0105.9459464
TTACTGG602.9920375E-7102.0833364
TATGGGC501.4881733E-598.07
TATATGG501.4881733E-598.05
Produced by FastQC (version 0.11.2)