FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_289.3510000005a167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_289.3510000005a167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166245
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA24761.4893681012962796No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC16671.0027369244187794No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC14970.9004782098709736No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT14640.8806279888116936No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT11750.7067881740804235No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA10430.6273872898433035No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT8700.523324009744654No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT8000.4812174802249692No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA7270.4373063851544407No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA5410.3254233210021354No Hit
GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTG5270.3170020150981984No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG5220.3139944058467924No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5020.30196396884116816No Hit
TGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAAC4460.26827874522542033No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA4240.25504526451923365No Hit
ATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT4180.2514361334175464No Hit
TCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT4130.24842852416614034No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT4100.2466239586152967No Hit
AAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATG3860.2321874342085476No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3720.22376612830461068No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3700.22256308460404825No Hit
AATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA3680.22136004090348582No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC3630.21835243165207974No Hit
TTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT3580.21534482240067368No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3510.21113416944870522No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3420.20572047279617434No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3330.20030677614364342No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC3200.19248699208998765No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3150.18947938283858162No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG3090.18587025173689437No Hit
ATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTT3010.18105807693464465No Hit
GTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATT2980.17925351138380102No Hit
ATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAA2960.1780504676832386No Hit
CAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGAT2700.1624108995759271No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTTCC2680.16120785587536468No Hit
ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGG2650.15940329032452105No Hit
ATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTT2590.15579415922283377No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTAATTAATGCA2590.15579415922283377No Hit
GAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTA2580.15519263737255254No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2500.15038046257030288No Hit
TTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAA2470.14857589701945925No Hit
AGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTG2440.1467713314686156No Hit
CCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGT2430.1461698096183344No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2400.14436524406749074No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT2400.14436524406749074No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2270.136545460013835No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2260.1359439381635538No Hit
AATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGA2200.1323348070618665No Hit
GCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC2180.13113176336130408No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2160.12992871966074168No Hit
ATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAA2050.12331197930764835No Hit
ATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGT2040.12271045745736714No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1980.11910132635567987No Hit
CTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTT1930.1160937171042738No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCCATCGTTCCATCAGGCCCC1920.1154921952539926No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1880.11308610785286777No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1870.11248458600258654No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1810.10887545490089927No Hit
CACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGC1810.10887545490089927No Hit
GCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTG1800.10827393305061807No Hit
ATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCC1750.10526632379921201No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT1740.1046648019489308No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1710.10286023639808717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3550.0141.478871
CGAAAGC3600.0139.51393
GCGAAAG3850.0130.454542
GAGCGAA1450.0118.275861
GCAGGGG757.4578566E-11114.3333369
GCAAAAG7400.0102.635133
TAGAAAC6300.0101.111112
CAAAAGC8100.093.7654344
CAGTATG1700.093.676479
GGTCGTT403.7445014E-491.875017
TTATATT1650.089.090912
TAGGCAT552.3924807E-589.090919
GGTAGTT552.3924807E-589.090917
GAGCAAA8700.087.298851
GCAGGGT6450.085.4651268
AAGCAGG10850.082.419367
GCTTATA603.6839854E-581.6666642
CAAGGGT6750.081.6666648
AGCAGGG7350.081.6666648
GTAGAAA7850.081.14651
Produced by FastQC (version 0.11.2)