FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_287.3510000005a2ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_287.3510000005a2ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150416
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5740.381608339538347No Hit
CCTGTAACCGTACCAACCATCCACCATTCCTTCCCAACCATTTTCTATGA4530.3011647697053505No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3720.2473141155196256No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3700.24598446973726198No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3290.21872673119880864No Hit
GGCTGGTCCAACCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT3090.20543027337517286No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2900.19279863844271886No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2890.19213381555153705No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC2800.18615040953090098No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2670.17750771194553772No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2650.17617806616317414No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2640.17551324327199233No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2370.15756302521008403No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2260.15024997340708438No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2220.14759068184235719No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2180.14493139027763002No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCAT2170.14426656738644825No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC2110.1402776300393575No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2050.1362886926922668No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG2000.13296457823635785No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1980.13163493245399424No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1910.12698117221572172No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1880.12498670354217636No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1880.12498670354217636No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1810.12033294330390384No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1760.1170088288479949No Hit
TCCCTGTACACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA1760.1170088288479949No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA1680.11169024571854058No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1640.10903095415381342No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1540.10238272524199553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC100.00147493245.000022
AGCGTCA100.00147493245.000023
CTAACAG100.00147493245.000021
GCGTCAG100.00147493245.000024
CGTCAGA150.0049626967163.333335
TAACCGT251.1627303E-4147.05
GGCTGGT551.1423253E-9133.636371
GGGTATT302.4031944E-4122.57
GTCCAAC602.0918378E-9122.56
GTAACCG302.4031944E-4122.54
AGGGTTA354.437731E-4105.000019
CCAACCC706.1099854E-9105.000018
GCAGGGT759.8680175E-998.000017
AGGGTGT801.5448677E-891.875016
AGGGTAT407.545952E-491.875016
ATATTCA801.5448677E-891.875014
CGAAAGC954.9112714E-1090.263153
CTAGGTC552.3917382E-589.09091245
CAACCCT852.3530447E-886.470599
TCTATGC1600.084.218753
Produced by FastQC (version 0.11.2)