FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_285.3510000005a53d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_285.3510000005a53d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105981
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC13681.2907974070824015No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT8100.7642879384040535No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA7800.7359809777224219No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC7270.6859720138515394No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6960.6567214878138534No Hit
ATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5600.5283965993904568No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4060.38308753455808114No Hit
TTTTTAAACTATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCG3910.3689340542172654No Hit
GTACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3870.3651597927930478No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA3770.3557241392325039No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3420.3226993517706004No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT3080.29061812966475126No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3060.2887309989526425No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2760.26042403827101085No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2660.25098838471046697No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2650.2500448193544126No Hit
GTTCTTAAATGCCATAAGCACAACATTCCCTTATACTGGAGATCCTCCAT2650.2500448193544126No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2590.24438342721808626No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2550.2406091657938687No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2410.2273992508091073No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2400.22645568545305292No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG2330.2198507279606722No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACT2290.21607646653645463No Hit
ATGTCTGAAGTTCTTCCCTCCGTATTTATTTCATCCTAAGTGACGGGTTC2290.21607646653645463No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2250.2123022051122371No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2150.20286655155169322No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1880.17739028693822478No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1800.16984176408978968No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTGATGGCCATCC1800.16984176408978968No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1760.16606750266557213No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1760.16606750266557213No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1730.16323680659740897No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1690.15946254517319142No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1680.15851897981713706No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1640.15474471839291948No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTACTAATTGATGGCCATCCGAAT1620.1528575876808107No Hit
GCCTCCAGGACACAGTCTGTTTGTGCATATCCACTTGGTTCATTATCCTC1580.14908332625659318No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1580.14908332625659318No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1530.14436549947632124No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1510.14247836876421244No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1500.14153480340815808No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT1500.14153480340815808No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG1470.1387041073399949No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1460.13776054198394053No Hit
GAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACTCGCGAGATA1460.13776054198394053No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1450.13681697662788614No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1440.13587341127183175No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1420.13398628055972295No Hit
GTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATA1420.13398628055972295No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1390.1311555844915598No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT1380.13021201913550542No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1370.12926845377945104No Hit
GCAATAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGA1300.12266349628707032No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTAATCCTTCCAGACTCGA1290.12171993093101592No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1280.12077636557496156No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1280.12077636557496156No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1280.12077636557496156No Hit
CCGTATTTATTTCATCCTAAGTGACGGGTTCTTTTCCTGTCTCCCCGATG1270.11983280021890716No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATG1250.11794566950679838No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1250.11794566950679838No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1250.11794566950679838No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC1250.11794566950679838No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1200.11322784272652646No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCG1180.11134071201441768No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1160.1094535813023089No Hit
AAACTATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC1150.10851001594625452No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1140.10756645059020013No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1130.10662288523414575No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT1110.10473575452203698No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT1100.10379218916598258No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1090.1028486238099282No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATT100.0014743163245.000027
GCTAATC100.0014743163245.000023
GGTTAAA100.0014743163245.000021
TAATCGG100.0014743163245.000025
TAAATGC850.0172.941186
GCAGGGT950.0167.631599
GGAAGGG150.004960638163.33333245
GCAGGGG452.8194336E-10163.333339
CCTCCAG505.857146E-10147.02
TTAAATG1000.0147.05
GAGCGAA1850.0139.054061
AAGCAGG9650.0137.098437
CGAAAGC1900.0135.394744
CAAAAGC8250.0130.666664
AGCAGGT7500.0124.133338
GCGAAAG2100.0122.53
GGTAGTT501.00893885E-7122.57
GCAGGTC3450.0120.724639
AGCAAAA8900.0116.9943852
AGCAGGG2050.0113.536588
Produced by FastQC (version 0.11.2)