FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_282.3510000005a3ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_282.3510000005a3ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157651
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6800.43133250026958275No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3710.2353299376470812No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3360.2131290001332056No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3180.2017113751260696No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3110.19727118762329449No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3070.1947339376217087No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2940.18648787511655493No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2860.18141337511338337No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2830.17951043761219404No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2790.17697318761060826No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2760.1750702501094189No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2490.15794381259871487No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2460.15604087509752554No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2430.1541379375963362No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT2420.15350362509593976No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2410.15286931259554332No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2360.1496977500935611No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2270.1439889375899931No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2250.1427203125892002No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGGAAAAAAGACG2230.1414516875884073No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2200.13954875008721795No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2140.1357428750848393No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA2090.13257131258285706No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2020.12813112508008195No Hit
GAGCAAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATC1970.12495956257809972No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1960.12432525007770327No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1950.12369093757730683No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1920.1217880000761175No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1830.1160791875725495No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1790.11354193757096372No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1780.11290762507056727No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1780.11290762507056727No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1760.11163900006977438No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1750.11100468756937794No Hit
GTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATA1730.10973606256858505No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1720.1091017500681886No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1720.1091017500681886No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1650.10466156256541347No Hit
GTACACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCAGGCAT1610.10212431256382769No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC1600.10149000006343124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC100.0014749971245.000024
TAACCGT100.0014749971245.000025
GTAACCG100.0014749971245.000024
GCAGGGG503.6379788E-12171.499989
CGAAAGC1400.0166.253
AAGGTAC150.004962922163.333335
GCAGGGT1000.0122.499999
GCAAAAG6000.093.916673
AGCGAAA2500.093.11
GCGAAAG2750.084.636362
TAATGGG801.6614722E-676.56254
AGCAAAA7650.075.261442
TAGAAAC2500.073.53
CAAGCTA504.0052597E-573.499994
ACAAGGT1007.264498E-873.499993
AAGCTAA504.0052597E-573.499995
AAGCAGG7700.073.1818167
GTATTAA707.90745E-570.01
CTAAATT557.6674485E-566.8181848
GCTAAAT557.6674485E-566.8181847
Produced by FastQC (version 0.11.2)