FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_281.3510000005a15b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_281.3510000005a15b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147736
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA13690.9266529485027345No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA13560.9178534683489469No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA7830.529999458493529No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA6050.40951426869551094No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5680.38446959441165324No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC5520.37363946499160666No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT5270.3567173877727839No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5150.34859479070774896No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT5020.3397953105539611No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4990.3377646612877024No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC4770.32287323333513834No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC4580.31001245464883304No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4520.30595115611631557No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG4460.30188985758379816No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4240.28699842963123406No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4230.28632154654248115No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3800.25721557372610604No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3760.2545080413710944No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG3680.24909297666107108No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3630.24570856121730655No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT3630.24570856121730655No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3610.2443547950398007No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3390.22946336708723672No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT3340.22607895164347214No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3300.2233714192884605No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG3270.22134077002220176No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3190.21592570531217847No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT3150.21321817295716683No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA3100.2098337575134023No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3060.20712622515839063No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3010.20374180971462608No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2910.19697297882709697No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2820.1908810310283208No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2790.18885038176206206No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2730.1847890832295446No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2690.18208155087453295No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2600.17598960307575676No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT2560.1732820707207451No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2490.16854388909947474No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2440.16515947365571018No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2400.16245194130069854No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2390.16177505821194565No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC2380.16109817512319272No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2370.1604212920344398No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT2360.1597444089456869No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2250.1522986949694049No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA2210.14959116261439323No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT2130.14417609790436997No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT2060.1394379162830996No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2050.13876103319434668No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2040.13808415010559377No Hit
GCATTGAATACTGACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGA2010.136053500839335No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT2000.13537661775058213No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1980.1340228515730763No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA1960.13266908539557046No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1950.13199220230681757No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC1940.13131531921806464No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC1930.13063843612931175No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1910.12928466995180593No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1870.12657713759679426No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA1840.12454648833053554No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT1790.121162072886771No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG1790.121162072886771No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1770.11980830670926518No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA1750.11845454053175934No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG1750.11845454053175934No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1720.11642389126550062No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1690.11439324199924189No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT1690.11439324199924189No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1660.11236259273298317No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG1660.11236259273298317No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1650.11168570964423025No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT1650.11168570964423025No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1640.11100882655547735No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1620.10965506037797151No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA1600.10830129420046569No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT1590.1076244111117128No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC1590.1076244111117128No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC1580.10694752802295987No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1570.10627064493420697No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG1560.10559376184545405No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT1540.10423999566794823No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT1530.10356311257919532No Hit
TAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGA1500.1015324633129366No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1500.1015324633129366No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1490.10085558022418367No Hit
TTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAG1490.10085558022418367No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1480.10017869713543077No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC100.0014749032245.00002245
GCTTATA204.777917E-5183.750022
GTAACCG204.777917E-5183.750024
GCAGGGG600.0163.333339
GATTAGA150.0049626064163.33333245
TATCCTA150.0049626064163.333335
GGTCGTT251.1626884E-4147.07
TAACCGT251.1626884E-4147.05
TAGGCAT251.1626884E-4147.09
AGGTCGT251.1626884E-4147.06
GAGCGAA2450.0135.01
GCAAAAG10000.0132.33
GCAGGGT5050.0130.99019
AGCAGGG5950.0129.705898
AGCTTAT302.4031075E-4122.51
TATAGGC302.4031075E-4122.57
ACAAGGT1150.0117.173913
AAGCAGG14400.0114.843757
CAAAAGC11900.0111.176474
TACGGAT901.8189894E-12108.888892
Produced by FastQC (version 0.11.2)