FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_279.3510000005a4b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_279.3510000005a4b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117223
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT7540.6432184810148179No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7250.6184793086680941No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4470.38132448410294906No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3480.2968700681606852No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2780.23715482456514508No Hit
GTATTGGGGAGTCCCCCAAAGGAGTGGAAGAAGGTTCCATTGGGAAAGTC2640.22521177584603705No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2540.21668102676095988No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2540.21668102676095988No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2490.2124156522184213No Hit
CAAATAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGA2470.21070950240140587No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2410.20559105295035957No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2410.20559105295035957No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2380.20303182822483642No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2310.1970603038652824No Hit
ATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2280.19450107913975928No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2100.17914573078662036No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2080.17743958096960494No Hit
GTATCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGG1930.1646434573419892No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1900.16208423261646607No Hit
ACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCTGCTGT1890.16123115770795832No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1860.1586719329824352No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1800.1535534835313889No Hit
CTCCAACTCTATGCTGACAAAATGACTGCCGTCAGCATCCACAGCATTCT1770.15099425880586573No Hit
ACAAATAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTG1740.1484350340803426No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1720.14672888426332717No Hit
CCCCAATACTGTCCAGGCCATTGTTCTGTCTCTCCCCTCGATTATTTCAA1690.14416965953780403No Hit
ATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGA1680.1433165846292963No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1680.1433165846292963No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1670.14246350972078858No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1630.13905121008675772No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1550.132226610818696No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT1540.13137353591018827No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1540.13137353591018827No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1500.1279612362761574No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1450.12369586173361882No Hit
CAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGT1450.12369586173361882No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1430.1219897119166034No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTGTTGTATA1410.12028356209958797No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG1410.12028356209958797No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1380.11772433737406482No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1370.11687126246555711No Hit
GTAGTAAACAGTATCTAAGTCAGTATTCAATAGCCTGTATGCATCACCAC1360.11601818755704939No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1330.11345896283152623No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCTTGTGCAT1270.10834051338047994No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1270.10834051338047994No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1270.10834051338047994No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1250.10663436356346452No Hit
CATATACAACAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1250.10663436356346452No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1220.10407513883794137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG304.776666E-9204.166679
GCAGGGT1150.0170.434789
GCTAAAT150.004961306163.333337
TTCGGCA251.1620804E-4146.999984
GTTCGGC302.401853E-4122.53
TGTTCGG302.401853E-4122.52
GCAGGTA3700.0119.1891869
GCAAAAG6550.0117.8244253
GAGCGAA1150.0117.173921
AAGCAGG7750.0115.38717
CAAAAGC7150.0107.9370654
AGCAGGT5200.0106.009618
GTGTTCG354.435261E-4105.01
GCAAGCC354.435261E-4105.08
CGGCAAG354.435261E-4105.06
AGGGTGT354.435261E-4105.06
TCGGCAA354.435261E-4105.05
AGCAGGG2450.0100.08
GCGAAAG1300.094.230773
TACCACG407.5417594E-491.875011
Produced by FastQC (version 0.11.2)