FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_264.3510000005a506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_264.3510000005a506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136669
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5370.3929201208759851No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3050.22316692154036394No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2850.20853302504591384No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2810.20560624574702382No Hit
GGCTGGTCCAACCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT2810.20560624574702382No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2790.20414285609757882No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2740.20048438197396629No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2710.19828929749979876No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2310.1690215045108986No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2270.16609472521200858No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2220.16243625108839604No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2180.15950947178950603No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2170.15877777696478354No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2120.155119302841171No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2070.15146082871755848No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2060.150729133892836No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2050.14999743906811347No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2050.14999743906811347No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1960.14341218564561095No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1880.1375586270478309No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1850.13536354257366337No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1840.13463184774894088No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1790.13097337362532835No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1780.13024167880060583No Hit
GAGCAAAAGCAGGTAGAGGTGGGAGCATGTGAAGGTGAGAGGTTATAGTA1730.1265832046769933No Hit
CCCCCAACACTGACCTCTGCGCTTATTCACTCATGCCCCTGCCTTAGGGC1710.1251198150275483No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1710.1251198150275483No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1700.12438812020282582No Hit
GTATTAAGTAGATCTCAATAAGAAAGTGATTTTTAAACAAGTATTTGAAG1610.11780286678032327No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1570.11487608748143324No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1560.11414439265671074No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1540.11268100300726573No Hit
GTATAAGAGAAAACAGAAAATGTATTCAGTAATAACTAAAAACCACGTAC1530.11194930818254323No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1530.11194930818254323No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC1480.1082908340589307No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1480.1082908340589307No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1460.10682744440948569No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA1450.10609574958476319No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1430.10463235993531816No Hit
GTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCGCCCGCCTC1430.10463235993531816No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1410.10316897028587316No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGAAATGGTTTG1390.10170558063642815No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1370.10024219098698314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT550.0178.181829
GACAGTA150.004962202163.333347
CAGTCTA150.004962202163.333349
ACGAACG150.004962202163.33334245
AGCAGGT2750.0138.090918
AAGCAGG6500.0130.038477
GCTTATA404.914473E-6122.500012
TAGGCAT404.914473E-6122.500019
TACATTG950.0116.052636
AGCAGGG3100.0110.645168
CGAAAGC1700.0108.0882344
AGCAAAA6150.0105.569112
ATTGTCT852.0372681E-10100.8823558
GCAAAAG6600.098.3712163
CAAAAGC7150.092.517484
AGCTTAT552.3909526E-589.090911
GGACAGT552.3909526E-589.090916
GCAGGGA1203.45608E-1181.666679
TATAGGC603.6816353E-581.666677
AGGTCGT300.00339204781.666676
Produced by FastQC (version 0.11.2)