FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_250.3510000005a2aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_250.3510000005a2aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38096
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5201.364972700545989No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4051.0631037379252415No Hit
GGATATAGGTAAAGAAGACAGAAGATACGGACCAGCATTAAGCATCAATG3150.8268584628307435No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2990.7848593028139438No Hit
GGATGAAATGAGAGGGTTCCTCATTATAGGTAAAGAAGACAGAAGATACG2050.5381142377152457No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCA1830.4803653926921461No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT1600.41999160016799664No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1220.32024359512809747No Hit
CCTATATCCTAAGTGACGGGTTCTTTTCCTGTCTCCCCGATGTGTACTTC1130.29661906761864765No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT1120.2939941201175977No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1110.29136917261654766No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGA1040.2729945401091978No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1020.2677446451070979No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA950.249370012599748No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA840.22049559008819825No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA790.20737085258294832No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC790.20737085258294832No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG770.20212095758084836No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG770.20212095758084836No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT720.18899622007559846No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA710.1863712725745485No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG690.18112137757244856No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG670.1758714825703486No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG660.17324653506929863No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG650.17062158756824863No Hit
GAATACTGACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTT620.1627467450650987No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG620.1627467450650987No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC600.15749685006299874No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATCACTGCTGACAAAAGG590.15487190256194877No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT580.1522469550608988No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT570.1496220075598488No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC560.14699706005879884No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC550.14437211255774884No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT540.14174716505669888No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT530.1391222175556489No Hit
GAATAAAAGAACTACGAAATCTAATGTCGCAGTCTCGCACTCGCGAGATA520.1364972700545989No Hit
CCTATAATGAGGAACCCTCTCATTTCATCCTAAGTGACGGGTTCTTTTCC520.1364972700545989No Hit
GTATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCAGTCTCGCACT510.13387232255354894No Hit
CCTATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCT490.12862242755144898No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT480.125997480050399No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT470.12337253254934902No Hit
GCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGA460.12074758504829904No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC460.12074758504829904No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA460.12074758504829904No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG450.11812263754724907No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG440.11549769004619909No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG440.11549769004619909No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC420.11024779504409912No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC420.11024779504409912No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA420.11024779504409912No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG410.10762284754304914No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT410.10762284754304914No Hit
AGTAATGACTGATGGAAATGCTACAGGAAAAGCTGATACTAAAATACTAT410.10762284754304914No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC400.10499790004199916No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT400.10499790004199916No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTTG100.0014706136244.99998245
TATTAGA100.0014706136244.999982
GCAGGGG254.690337E-7195.999989
AAGGTAC204.7502213E-5183.749989
CAAGGTA204.7502213E-5183.749988
GAGCGAA700.0175.01
AGCAGGG850.0172.941188
CCGCTAT150.0049482174163.33333245
GCAGGGT600.0163.333339
AAGGGTG150.0049482174163.333335
TAGCTTT150.0049482174163.33333245
ACAAGGT301.1631728E-6163.333337
TCTATGC150.0049482174163.333333
AAGCAGG3800.0157.960537
CAAAAGC3400.0144.117654
AGCAGGT3100.0138.306448
CGAAAGC900.0136.11114
AGCAAAA3550.0134.577452
GCAGGTA2100.0134.166669
GCAGGTC1200.0122.59
Produced by FastQC (version 0.11.2)