FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_247.3510000005a223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_247.3510000005a223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130640
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT24091.8439987752602571No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA11150.8534905082669932No Hit
GCCATGGAACAGGGACAGGGTACACTATGGACACAGTCAACAGAACACAC10700.8190447030006124No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG6800.5205143906919779No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6770.5182180036742192No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA6350.48606858542559706No Hit
GTGTTTACCTGGGTGGGATTCTTCAAGGAAGGCCATAGCCTCCAGGACAC6160.47152480097979177No Hit
GCCATGGACACAGTCAACAGAACACACCAATATTCAGAGAGGGGGAAGTG5960.4562155541947336No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4930.377372933251684No Hit
GCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC4840.37048377219840783No Hit
GAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT4790.3666564605021433No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC4750.36359461114513164No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4300.3291488058787508No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3680.28169014084507044No Hit
GCCCAATTAGCACATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCA3530.2702082057562768No Hit
ATTCTGCACAAACAGACTGTGTCCTGGAGGCTATGGCCTTCCTTGAAGAA3280.25107164727495407No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3030.23193508879363134No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3000.22963870177587264No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTGT2980.2281077770973668No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTAAAAG2960.226576852418861No Hit
TATCCACTTGGTTCATTATCCTCAGGTAGTGGTCCATCAATTGGGTTGAG2920.22351500306184935No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2830.21662584200857318No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2820.21586037966932026No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2800.21432945499081446No Hit
GTCCAGGCCATTGTTCTGTCTCTCCCTTCGATTATTTATTGATTCGCCTT2690.20590936925903247No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2610.19978567054500918No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA2600.1990202082057563No Hit
TAGTAGCACTGCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2570.19672382118799756No Hit
AGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTT2530.1936619718309859No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2500.19136558481322719No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2480.1898346601347214No Hit
AGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTT2390.1829454990814452No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2230.17069810165339866No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA2200.16840171463563994No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2160.1653398652786283No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2060.1576852418860992No Hit
GTATACTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2030.15538885486834048No Hit
GTCCCTAAGAGCCTGAACAACAAGGAGCAGTTTTCTTGACTCCGCTGAAA2020.15462339252908758No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGGTTGTCCCT2000.15309246785058175No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1990.15232700551132886No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1970.15079608083282303No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1950.1492651561543172No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1950.1492651561543172No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA1940.1484996938150643No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1890.14467238211879976No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1870.14314145744029394No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1860.14237599510104104No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA1820.1393141457440294No Hit
GTTTACCTGGGTGGGATTCTTCAAGGAAGGCCATAGCCTCCAGGACACAG1770.13548683404776485No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1770.13548683404776485No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGATCA1750.13395590936925902No Hit
CTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGA1720.1316595223515003No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAAACGCATTTGAAAAAAAGACG1690.12936313533374158No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT1670.12783221065523576No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1670.12783221065523576No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1630.12477036129822414No Hit
GCCCAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC1590.12170851194121249No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1540.11788120024494794No Hit
GGTAAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGA1520.11635027556644213No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1510.1155848132271892No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT1490.1140538885486834No Hit
CTTAGGGACAACCTCGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1460.11175750153092467No Hit
GCTCTATTCTGCACAAACAGACTGTGTCCTGGAGGCTATGGCCTTCCTTG1460.11175750153092467No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1450.11099203919167178No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1450.11099203919167178No Hit
CTGTCCAGGCCATTGTTCTGTCTCTCCCTTCGATTATTTATTGATTCGCC1420.10869565217391304No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1410.10793018983466013No Hit
ACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTCAGGAAGGAG1410.10793018983466013No Hit
TCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1390.10639926515615432No Hit
ATTCTAAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA1380.10563380281690139No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGGTTGTCCCTAAGAG1360.10410287813839558No Hit
CCTCCAAGTGAGTGCATATTGCTGCAAATTTGTTGGTTTCAATTTTCAGA1350.10333741579914268No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1350.10333741579914268No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1340.10257195345988977No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCAT1340.10257195345988977No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1330.10180649112063686No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1330.10180649112063686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAAT100.0014747082245.00002245
GGTCGTT150.0049619526163.333337
TAGGCAT150.0049619526163.333334
AGGTCGT150.0049619526163.333336
GAGCGAA2300.0154.456511
AGCAGGT9300.0147.526898
GCAGGTA7850.0142.006369
AAGCAGG13600.0141.415447
CGAAAGC2450.0140.000024
GCAAAAG11200.0140.03
GCGAAAG2600.0131.923083
AGCAAAA12300.0129.471542
GGTACTT302.4024765E-4122.57
CAAAAGC12900.0122.54
GCAGGGT2000.0122.59
ACAAGGT757.4578566E-11114.3333363
TAAACTA653.6452548E-9113.076925
AGCGAAA3150.0108.888892
AAAGCAG18200.0105.06
GAGCAAA15200.0103.963811
Produced by FastQC (version 0.11.2)