FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_238.3510000005a25a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_238.3510000005a25a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200487
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG3900.19452632839036943No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3780.188540902901435No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCAT3520.17557248100874373No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3460.17257976826427648No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3150.15711741908452917No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2950.1471417099363051No Hit
GTTTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATAAGA2940.14664292447889388No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2860.14265264081960427No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2820.14065749898995944No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2730.1361684298732586No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2610.13018300438432417No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG2550.12719029163985696No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2480.12369879343797853No Hit
TCCCTGTACACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA2300.11472065520457685No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2220.11073037154528723No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2090.10424616059894157No Hit
GTATGTAATCATCGCCAAAAACATTCGGGGGTTTTGATTTTCATTCCACT2090.10424616059894157No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2090.10424616059894157No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA2090.10424616059894157No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG2060.10274980422670797No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2050.10225101876929676No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC2050.10225101876929676No Hit
AAACTACACATACCCAGCATTGAACGTGACTATGCCAAACAATGAACAAT2030.10125344785447436No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2020.10075466239706314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTT254.7402318E-7196.07
AGGTCGT254.7402318E-7196.06
GGGTTAA301.1755237E-6163.333331
ACCGAGG653.6561687E-9113.076926
CTAACCG653.6561687E-9113.076923
GAGGTCG653.6561687E-9113.076929
CGAGGTC653.6561687E-9113.076928
CGAAAGC801.2551027E-10107.187513
CCGAGGT706.1208993E-9105.07
TTCTAAC852.0372681E-10100.8823551
AACCGAG759.884388E-998.000015
TAACCGA759.884388E-998.000014
GTACTGA501.491345E-598.06
GCAAACC407.5496477E-491.875018
GCAGGGG407.5496477E-491.875018
TTTAAAC552.3936891E-589.090913
GTCGTAC552.3936891E-589.090911
CGTACTC552.3936891E-589.090913
TCCAACT1600.084.218752
GGCAAAC450.001205373881.666677
Produced by FastQC (version 0.11.2)