FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_235.3510000005a4a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_235.3510000005a4a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134665
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA14731.0938254186314187No Hit
TAATCAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC11170.8294657112092971No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10420.773771952623176No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4660.34604388668176583No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC4430.32896446738202206No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3830.28440946051312516No Hit
AAACAAGGGTGTTTTTAATCAATGCACTCAAATGCAAATGTTGCACCTAA3750.27846879293060556No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3730.27698362603497567No Hit
GTTTTTAATCAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT3480.25841903983960196No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3480.25841903983960196No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3420.2539635391527123No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3280.24356737088330302No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3020.22426020124011437No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3020.22426020124011437No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT3020.22426020124011437No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA2910.21609178331414994No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT2740.20346786470129583No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2690.19975494746222108No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2670.1982697805665912No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2650.19678461367096128No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2610.19381427987970148No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2540.18861619574499683No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2420.17970519437121746No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2360.17524969368432777No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2350.17450711023651283No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2330.17302194334088292No Hit
GTAGAAACAAGGGTGTTTTTAATCAATGCACTCAAATGCAAATGTTGCAC2270.16856644265399323No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2210.16411094196710355No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC2190.16262577507147366No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2160.1603980247280288No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2060.15297219024987932No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2010.14925927301080458No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT1900.14109085508484015No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1900.14109085508484015No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1830.13589277095013552No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1830.13589277095013552No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1740.12920951991980098No Hit
TTTTTAATCAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT1740.12920951991980098No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1670.12401143578509635No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1660.12326885233728141No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1660.12326885233728141No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT1650.12252626888946645No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC1610.11955593509820667No Hit
AATCAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA1610.11955593509820667No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1560.11584301785913192No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1540.11435785096350203No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC1540.11435785096350203No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1520.11287268406787214No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT1510.11213010062005718No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT1490.11064493372442728No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1490.11064493372442728No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT1480.10990235027661234No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1470.1091597668287974No Hit
TCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAATCCATTAAT1440.10693201648535255No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1430.1061894330375376No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1410.1047042661419077No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC1390.10321909924627781No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG1350.10024876545501801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1100.0144.772721
GCAGGGT4600.0135.815229
GCAAAAG7050.0135.531923
CGAAAGC1350.0117.962964
ACAAGGT901.8189894E-12108.888893
CAAAAGC9050.0108.2872854
AGCAGGG6150.0107.560988
TAGTTTT801.2551027E-10107.187499
AAGCAGG10650.0104.6713647
AGCGAAA1550.0102.741932
GGTAGTT852.0372681E-10100.8823557
GCGAAAG1800.088.472223
CAACTCT1550.086.935484
CAAGGTA1300.084.807694
AGCAGGT4300.082.616288
GAGCAAA11750.082.36171
CTAGAGT450.001204509281.666676
AGGGTTA604.22204E-681.666679
AGGGTGT1800.081.666676
TTATATT450.001204509281.666672
Produced by FastQC (version 0.11.2)