FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_220.3510000005a421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_220.3510000005a421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183102
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7880.4303612194296076No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3150.17203525903594716No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2930.16002009808740483No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2800.15292023025417525No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2760.15073565553625848No Hit
TCCTACAGCACCCTGGAATGGCTGAAATTAAGTATTTTATATGGCAAAAT2750.15018951185677928No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2620.14308964402354973No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2450.13380520147240335No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2430.13271291411344496No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2370.12943605203656977No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2200.12015160948542342No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2190.11960546580594422No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2160.11796703476750663No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA2130.11632860372906904No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2050.11195945429323546No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA1980.10813644853688109No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1880.10267501174208911No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1840.10049043702417232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG8900.0126.629213
AGCAAAA9250.0123.162162
GAGCGAA2300.0122.51
GAGCAAA10350.0113.623191
CAAAAGC10700.0105.327114
TACGGAT354.4392896E-4105.02
ACGGATA354.4392896E-4105.03
AAGCAGG14350.097.317087
GCGAAAG2850.094.561413
CGGATAA407.548596E-491.875014
AGCGAAA3150.085.555552
CGAAAGC3200.084.218754
AAAAGCA13900.081.079145
AAAGCAG17750.079.3661966
GCAGGTA4850.078.2989659
GCTTATA500.001830950573.52
AGCAGGG7000.071.758
TAGAAAC2400.066.3541642
AGCAGGA3200.065.0781258
AGCAGGT7550.064.9006658
Produced by FastQC (version 0.11.2)