FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_203.3510000005a2b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_203.3510000005a2b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145556
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT13270.9116766055676165No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10440.7172497183214708No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG9740.6691582621121768No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC8070.554425788012861No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5790.3977850449311605No Hit
TAATTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT5130.35244167193382614No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4570.31396850696639095No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4100.28167852922586495No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4100.28167852922586495No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT4040.2775564044079255No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC3880.2665640715600868No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3820.2624419467421474No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3590.24664046827337932No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3350.23015196900162135No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3300.22671686498667182No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC3180.21847261535079282No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC3130.21503751133584323No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC3000.20610624089697438No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2940.20198411607903485No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT2920.20061007447305504No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2850.19580092885212566No Hit
CTATTAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC2730.18755667921624666No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2670.18343455439830716No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2420.16625903432355932No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2400.16488499271757948No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2260.1552667014757207No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2230.15320563906675094No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2190.1504575558547913No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2180.14977053505180138No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2170.14908351424881144No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2170.14908351424881144No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2100.14427436862788204No Hit
GTGTCAACAGTGCATTTGGATCGTCAAGTTCTACTACTATTGATTCGCCT2090.14358734782489213No Hit
GTGTAATTATGGCCATATGGTCCACTGTGGTTTTTGTCAGTATCTCGCGA2090.14358734782489213No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2050.14083926461293247No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2040.14015224380994257No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2000.1374041605979829No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1940.1332820357800434No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1940.1332820357800434No Hit
GCAATGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA1940.1332820357800434No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1830.12572480694715435No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1820.12503778614416444No Hit
ATTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGG1810.12435076534117454No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1800.12366374453818463No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1780.1222897029322048No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1750.12022864052323504No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1740.11954161972024513No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1670.11473247409931574No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1650.11335843249333591No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1650.11335843249333591No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1610.11061034928137624No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1600.10992332847838632No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1580.10854928687240649No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1570.10786226606941658No Hit
CTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG1560.10717524526642667No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1560.10717524526642667No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA1530.10511418285745693No Hit
ATCCCATATTGTCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1530.10511418285745693No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1490.10236609964549725No Hit
GGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGATGCA1480.10167907884250735No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1470.10099205803951743No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1460.10030503723652752No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAACT100.0014748812245.000024
GTTATGC100.0014748812245.000021
GCAGGGT1700.0165.73539
GAGCGAA1300.0160.19231
GCAGGGG1550.0142.258069
AAGCAGG12450.0139.718877
CAAAAGC11400.0134.320184
TTTAAAC551.1405064E-9133.636373
AGCAGGT7750.0132.77428
GCAAAAG11600.0132.004323
AGCAGGG3950.0127.15198
GCAGGTA6850.0126.970819
AGCAAAA12100.0126.549592
CGAAAGC1950.0119.358983
GAGCAAA13400.0114.2723851
CTAAATT551.7845741E-7111.363643
GCTAAAT551.7845741E-7111.363642
CGTACTC458.827994E-6108.888883
GCGAAAG2200.0105.7954562
AGCGAAA2300.0101.195651
Produced by FastQC (version 0.11.2)