FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_171.3510000005a124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_171.3510000005a124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156958
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA14350.9142573172441035No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT10660.6791625785241913No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT8150.5192471871456058No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5650.3599689088800826No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG5570.3548720039755858No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4380.27905554352119677No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4260.2714101861644516No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4240.27013595993832745No Hit
TTTTTGGACAGTACGGATAACAAATAGAAGCACTGCTATAACTATTTTAA4000.2548452452248372No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3360.21407000598886328No Hit
GTACGGATAACAAATAGAAGCACTGCTATAACTATTTTAATGCATGTGTC3210.20451330929293185No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3060.19495661259700045No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2900.18476280278800697No Hit
GCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGA2730.1739318798659514No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2720.17329476675288932No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2720.17329476675288932No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2680.17074631430064094No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2650.16883497496145466No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2620.16692363562226836No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2600.16564940939614417No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2580.16437518317002No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCTACAGCATTCTGCTGT2540.16182673071777162No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2440.1554555995871507No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2420.15418137336102652No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2390.15227003402184025No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2320.1478102422304056No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2290.14589890289121932No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2270.14462467666509513No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2250.14335045043897093No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCTACAGCATTCT2230.14207622421284677No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2220.14143911109978466No Hit
GCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGGCG2190.13952777176059838No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2160.1376164324214121No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2020.1286968488385428No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1960.12487417016017023No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1950.12423705704710813No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1890.12041437836873559No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1890.12041437836873559No Hit
CTATTAATGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGCAT1840.11722881280342512No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA1830.11659169969036304No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1830.11659169969036304No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1730.1102205685597421No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1700.10830922922055583No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1690.10767211610749373No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1680.10703500299443164No Hit
AGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTG1670.10639788988136953No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT1650.10512366365524534No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1640.10448655054218325No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1620.10321232431605906No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1580.10066387186381069No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1570.1000267587507486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTGT351.2021701E-8174.999986
ACGCCAA150.0049629016163.33333245
GGGTATT150.0049629016163.333337
ATGTAGG150.0049629016163.333336
TATGTAG150.0049629016163.333335
GCAGGGT1800.0142.916679
GCAGGGG1700.0136.911769
GCAAAAG8800.0136.420463
AAGCAGG11800.0120.423737
CGAAAGC1750.0119.000014
AGCAGGT6400.0114.843768
CAAAAGC10750.0111.6744164
GTCGTAC551.785229E-7111.363641
AGCAAAA10900.0110.137622
TAGATGG901.8189894E-12108.888898
AAGGTAC354.4380967E-4104.999999
GAGCAAA11800.0102.775421
AGCAGGG4800.0102.0833368
GCAGGTA6950.0100.467619
GCGAAAG2100.099.1666643
Produced by FastQC (version 0.11.2)