FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_168.3510000005a29d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_168.3510000005a29d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170639
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA18701.095880777547923No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG10760.6305709714660775No Hit
GAGCAAAAGCAGGGGATAATTCTATCAACCATGAAGACTATCATTGCTTT7680.4500729610464197No Hit
ATTAAAGTGCAGTTTTTTCCATCAAGGATCTGATGAGGACTGTCGCATAT7240.42428753098646854No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG6630.3885395484033544No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6620.3879535159019919No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5500.3223178757493891No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC5090.2982905431935255No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4730.27719337314447456No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC4290.25140794308452347No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4270.2502358780817984No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT4190.2455476180708982No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC3980.2332409355422852No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3820.22386441552048478No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3690.21624599300277192No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3640.2133158304959593No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3610.21155773299187172No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3540.20745550548233405No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3360.19690692045780858No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG3340.1957348554550835No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3280.19221866044690838No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3160.18518627043055808No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3090.1810840429210204No Hit
TGAAAACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAA3030.17756784791284524No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2820.16526116538423222No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2760.16174497037605706No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT2680.15705671036515687No Hit
CCTGTATATGGGTCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2620.15354051535698168No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATGGACGCATTTGAAAAAAAGACG2600.15236845035425667No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2590.15178241785289412No Hit
GTATATGGGTCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT2570.15061035285016908No Hit
GAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGTCTGCAAATTTT2510.14709415784199392No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2500.1465081253406314No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2450.14357796283381877No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2410.14123383282836866No Hit
GTTATATATCGCGAAGCAGGTGTTGAGACCATGGTCATTTTAAGTGCCTC2380.1394757353242811No Hit
ATATATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC2370.13888970282291854No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA2340.13713160531883098No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT2340.13713160531883098No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2320.13595954031610594No Hit
ATATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTA2280.13361541031065582No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2280.13361541031065582No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2260.13244334530793078No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2260.13244334530793078No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT2260.13244334530793078No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2260.13244334530793078No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2120.12423889028885542No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2110.12365285778749288No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC2100.12306682528613036No Hit
CACTCTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAA2100.12306682528613036No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA2030.11896459777659268No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2010.11779253277386763No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA1970.11544840276841754No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC1970.11544840276841754No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1950.11427633776569249No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT1940.11369030526432995No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA1910.11193220776024239No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC1900.11134617525887987No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT1880.11017411025615481No Hit
GCACTCTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAA1860.10900204525342976No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC1840.1078299802507047No Hit
GCAAAAGCAGGGGATAATTCTATCAACCATGAAGACTATCATTGCTTTGA1830.10724394774934219No Hit
GTGTTATCATTCCATTCAAGCCCTCCGATGAGGACCCCAATTGCATTTTT1820.10665791524797966No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT1800.1054858502452546No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGGCTTGAA1790.10489981774389208No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1770.10372775274116702No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1750.10255568773844198No Hit
GAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1720.1007975902343544No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAA100.0014751034244.99998245
GCAGGGT2900.0177.413799
GCAGGGG1700.0158.529429
CGAAAGC2100.0145.833334
AGCAAAA11750.0142.829792
AAGCAGG14850.0134.461297
AGCAGGG6000.0130.666678
CAAAAGC12850.0130.60314
GCAAAAG12950.0130.540543
GCGAAAG2550.0124.901963
GCAGGTA7700.0116.136369
GAGCAAA14650.0114.5563051
AGCAGGT8750.0113.400018
ATTGTCT1100.0100.227278
AGGGTGT654.8320544E-794.230776
AAAGCAG21600.091.8756
AAAAGCA18700.090.401075
GAGCGAA3600.085.069451
TCATGGT903.5011908E-881.66667245
ACTAATA450.001205064181.666678
Produced by FastQC (version 0.11.2)