FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_162.3510000005a216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_162.3510000005a216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135269
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT30012.2185423119857473No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA14241.0527171783631135No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT9770.7222645247617709No Hit
CTATAGGATGAACAGATGTACCAGAAGTGCTGCAACTTGTTCGAGAAATT8380.6195063170423378No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGA8230.6084173018208163No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA7580.5603649025275561No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5610.4147291692849064No Hit
GTTGACTGTGTCCATAGTGTACCCTGTCCCTGTTCCATGGCTGTATGGAG5600.4139899016034716No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4390.32453851214986434No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4260.3149280322912123No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4040.29866414329964736No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT4010.29644634025534305No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA4000.2957070725739083No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA3980.29422853721103875No Hit
GTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATA3670.27131123908656085No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTTC3630.26835416836082177No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3580.2646578299536479No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3580.2646578299536479No Hit
CTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGGGAAGTGG3520.2602222238650393No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3300.24395833487347432No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3250.24026199646630048No Hit
TCCTCAAGAATTCCCCTTTGGCTTGTGTTTAGAATAGAGCGGTTCCTTTT3200.23656565805912663No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2890.2136483599346487No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2800.2069949508017358No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2630.1944274002173447No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2600.19220959717304037No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2490.1840776526772579No Hit
ACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCTGGTACATCTGTT2450.18112058195151884No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2410.17816351122577975No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2360.17446717281860588No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2300.17003156672999725No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2290.1692922990485625No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2290.1692922990485625No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTT2240.16559596064138865No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2230.1648566929599539No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2220.1641174252785191No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2210.16337815759708432No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2160.15968181918991048No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC2150.1589425515084757No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2150.1589425515084757No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT2140.15820328382704094No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2120.1567247484641714No Hit
TCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA2110.15598548078273664No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2040.15081060701269322No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2000.14785353628695416No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1950.1441571978797803No Hit
GGCCTCCACCATGCTAGAAATTCCAATCGGTCTCCTATATGAACTACTAG1920.141939394835476No Hit
GATCTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAA1920.141939394835476No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1880.1389823241097369No Hit
TTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTAGACACCATG1880.1389823241097369No Hit
ATAGGATGAACAGATGTACCAGAAGTGCTGCAACTTGTTCGAGAAATTTT1760.13011111193251965No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT1750.1293718442510849No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTT1750.1293718442510849No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA1720.12715404120678056No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG1680.12419697048104147No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1630.12050063207386763No Hit
ATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGGGAAGTGGAC1630.12050063207386763No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1620.11976136439243285No Hit
ACTATAGGATGAACAGATGTACCAGAAGTGCTGCAACTTGTTCGAGAAAT1610.11902209671099809No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT1600.11828282902956332No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1590.11754356134812853No Hit
TCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATACT1570.116065025985259No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1550.11458649062238947No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT1540.11384722294095469No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1520.11236868757808516No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1500.11089015221521561No Hit
ATCCTATAGTGTACCCTGTCCCTGTTCCATGGCTGTATGGAGGATCTCCA1500.11089015221521561No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1490.11015088453378084No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA1480.10941161685234606No Hit
AAGCACAACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG1480.10941161685234606No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1430.10571527844517221No Hit
GCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGGCG1410.10423674308230267No Hit
TCCTATAGTGTACCCTGTCCCTGTTCCATGGCTGTATGGAGGATCTCCAG1400.1034974754008679No Hit
CCATGCTAGAAATTCCAATCGGTCTCCTATATGAACTACTAGGGAAAAAT1400.1034974754008679No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1390.10275820771943311No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1390.10275820771943311No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTTTGCCG1370.10127967235656359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG1000.0183.759
GCAAAAG12300.0172.296753
AAGGTAC150.0049621463163.333339
GCAGGGT3450.0163.333339
GCAGGAA150.0049621463163.333339
CAAAAGC13200.0161.477284
AAGCAGG15850.0157.66567
GAGCGAA2450.0150.01
AGCAGGG5050.0147.970298
CGAAAGC2500.0147.04
AGCAAAA15050.0141.627912
GAGCAAA15650.0140.111821
AGCAGGT6300.0138.055568
GCGAAAG2850.0128.947373
AAAGCAG20450.0122.200496
AAAAGCA17450.0122.1489945
AGCAGGA653.6470738E-9113.076928
AGCGAAA3350.0109.701492
GCAGGTA6500.0107.423089
AGCAGGC8050.0100.434788
Produced by FastQC (version 0.11.2)