FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_159.3510000005a513.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_159.3510000005a513.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109084
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7010.6426240328554143No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6490.5949543471086502No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4750.4354442448021708No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA3380.3098529573539658No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3210.29426863701367756No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3060.280517766125188No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2980.27318396831799346No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2810.25759964797770524No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2800.25668292325180597No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2760.25301602434820875No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2650.24293205236331633No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2630.24109860291151772No Hit
CTACTGTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCC2540.23284808037842397No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2460.22551428257122952No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2330.21359686113453852No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2280.20901323750504197No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2150.197095816068351No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2020.18517839463166No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1990.1824282204539621No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1950.17876132155036487No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1920.17601114737266693No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1920.17601114737266693No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1850.1695940742913718No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1850.1695940742913718No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGATTCGA1750.1604268270323787No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1730.1585933775805801No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1680.15400975395108357No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1680.15400975395108357No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1670.15309302922518425No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC1660.15217630449928496No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1610.14759268086978844No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1610.14759268086978844No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT1570.14392578196619119No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1570.14392578196619119No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1560.1430090572402919No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1540.14117560778849328No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1530.140258883062594No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1520.13934215833669464No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1510.13842543361079535No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1510.13842543361079535No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1510.13842543361079535No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1500.13750870888489605No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA1480.13567525943309744No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1480.13567525943309744No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC1390.12742473690000367No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA1370.12559128744820505No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1240.11367386601151407No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT1190.10909024238201753No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1170.10725679293021892No Hit
GATCTTCTTCTTTATCCTTCCAGATTCGAAGTCAATTCTGGCATCAATCC1160.10634006820431961No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1140.10450661875252099No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1120.10267316930072237No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT1110.10175644457482308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA800.0214.375021
GCAGGGG351.1998054E-8175.09
GAGTAAT150.0049608364163.333331
GTCGTAC150.0049608364163.333331
CGTACTC150.0049608364163.333333
TCGTACT150.0049608364163.333332
GCAGGGT1900.0154.736859
CGAAAGC1150.0149.130434
GCAAAAG7050.0133.794313
CAAAAGC7300.0130.890414
AAGCAGG8650.0130.289027
AGCAGGT4550.0129.230778
GCGAAAG1350.0127.037033
GCAGGTA4300.0119.651179
AGCAGGG3550.0100.070438
AGCAAAA9750.099.256422
GAGCAAA10150.095.3448261
AGCGAAA1800.095.277782
AAAGCAG12200.092.3770456
AAAAGCA10750.090.0232545
Produced by FastQC (version 0.11.2)