FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_153.3510000005a49b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_153.3510000005a49b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132369
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9610.7260008007917261No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5400.40795050200575667No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT4400.33240411274543136No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4260.32182761824898576No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3710.28027710415580687No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3470.2621459707333288No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3460.26139050684072557No Hit
CATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCT3220.24325937341824747No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3080.23268287892180195No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3060.23117195113659542No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3010.22739463167357915No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2990.22588370388837267No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2940.22210638442535638No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTT2900.21908452885494337No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2770.20926349825110108No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2690.20321978711027505No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2530.19113236482862303No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2460.18584411758040023No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2360.1782894786543677No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG2350.17753401476176447No Hit
TAGTAATACTATGGTCTCTAATCGGCCAAAAATCACATTGAAATTCGCTT2320.17526762308395472No Hit
GTTCTGGATCAACCGTCCTTCATGATCACTGAGTTTAAGTTCAGTGCACA2300.1737566952987482No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2260.1707348397283352No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2250.16997937583573192No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2200.16620205637271568No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2100.15864741744668315No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2030.15335917019846038No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1980.14958185073544408No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1960.14807092295023758No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1950.14731545905763435No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1940.14655999516503107No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1880.14202721180941155No Hit
ATCCAAAACAGCTTGACAATAGAGAAAATGGTACTCTCTGCTTTTGATGA1880.14202721180941155No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1850.13976082013160182No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC1800.13598350066858556No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1760.13296164509817254No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1690.12767339784994974No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1650.12465154227953675No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1630.12314061449433024No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1590.12011875892391723No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG1580.11936329503131399No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAGAATAA1570.11860783113871072No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1500.11331958389048796No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1450.10954226442747168No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1410.10652040885705868No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1400.10576494496445542No Hit
GTAATACTATGGTCTCTAATCGGCCAAAAATCACATTGAAATTCGCTTTT1400.10576494496445542No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1360.1027430893940424No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1350.10198762550143917No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG1330.10047669771623266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT1450.0177.413799
CGAAAGC1300.0150.769244
AGGGTGT455.3882104E-8136.111116
AGCAGGT4950.0123.737378
GTCGTAC404.9138944E-6122.500011
CGTACTC404.9138944E-6122.500013
GCAGGGG501.01035766E-7122.499999
AAGCAGG8600.0119.651157
GCAAAAG7100.0115.5985953
GAGCGAA1750.0112.000011
GCAGGTA4350.0109.827599
TCGTACT458.825013E-6108.888892
GCGAAAG1850.0105.9459463
TACTCTA354.4365416E-4105.03
CAAAAGC8050.0101.956524
AGCAGGG3200.099.531268
AGCAAAA8400.099.166672
TTTATTG501.4894631E-597.999992
AGCGAAA2050.095.6097642
TAGTTTT1051.0913936E-1193.3333369
Produced by FastQC (version 0.11.2)