FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_152.3510000005a414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_152.3510000005a414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172633
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9450.5474040305156025No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG7160.41475268343827654No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT5610.32496683716323066No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC5560.322070519541455No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4690.2716745929225583No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC4650.26935753882513774No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4410.2554552142406145No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3910.22649203802285775No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3480.20158370647558693No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3430.19868738885381126No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT3290.19057769951283937No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3260.18883990893977395No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3150.18246801017186748No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3140.18188874664751234No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC3100.1795716925500918No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3070.17783390197702642No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA2810.1627730503437929No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC2740.15871820567330697No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2640.1529255704297556No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGATCCCAATTGCATTTTT2610.1511877798566902No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGGCG2610.1511877798566902No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2530.14655367166184913No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2500.14481588108878374No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2490.14423661756442857No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2380.1378647187965221No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGATCCTCATCGGAGGACTTGAA2360.13670619174781184No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2330.13496840117474643No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2320.1343891376503913No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA2170.12570018478506428No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2160.12512092126070912No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2150.124541657736354No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2130.12338313068764374No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT2020.11701123191973724No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1980.1146941778223167No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1970.11411491429796157No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1950.1129563872492513No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT1930.11179786020054103No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1870.10832227905441022No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1850.10716375200569994No Hit
GCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACTGTGTTAT1850.10716375200569994No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT1810.10484669790827941No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1800.10426743438392429No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1790.10368817085956913No Hit
GTAGTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTT1790.10368817085956913No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1780.10310890733521401No Hit
ACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTA1740.10079185323779347No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1740.10079185323779347No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1740.10079185323779347No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT100.001475118245.000025
GTAACCG100.001475118245.000024
GCAGGGG1050.0163.333339
GCAGGGT2400.0117.3958369
AGGGTTA706.1154424E-9105.09
GCAAAAG6950.0100.467633
CGAAAGC1600.099.531263
AGCAGGG4700.099.042568
AGGGTGT1350.090.7407466
GAGCGAA1700.086.470591
AGCAAAA8350.083.622762
CAAAAGC9000.081.6666644
AAGCAGG10550.081.2796257
TTATATT500.001830771973.57
TAGAAAC2350.072.978722
AAGCCTC1900.070.921067
ACAAGGT707.9095735E-570.07
CTACAAG1950.069.102573
GCTTATA550.00267171766.8181842
AATTATA550.00267171766.8181845
Produced by FastQC (version 0.11.2)