FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_143.3510000005a3df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_143.3510000005a3df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149754
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9420.6290316118434232No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT4170.2784566689370568No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4050.27044352738491123No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3330.22236467807203814No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3020.2016640623956622No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2990.19966077700762586No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG2940.1963219680275652No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2620.17495359055517715No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2590.17295030516714077No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2590.17295030516714077No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2470.16493716361499525No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2350.15692402206284975No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2170.14490430973463148No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2100.1402299771625466No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2080.13889445357052232No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2060.13755892997849806No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2040.13622340638647382No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1940.12954578842635256No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1900.12687474124230402No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1900.12687474124230402No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1870.12487145585426766No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1790.11952936148617065No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1780.11886159969015853No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1770.11819383789414639No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1760.11752607609813426No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1720.11485502891408576No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1700.11351950532206151No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1650.11018069634200088No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1620.1081774109539645No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1580.105506363769916No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1570.10483860197390386No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1560.10417084017789173No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1550.10350307838187962No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA1530.10216755478985537No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1520.10149979299384324No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1510.1008320311978311No Hit
GCTATAACTACTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1510.1008320311978311No Hit
ATGTTTGGAGATTGCCTGTAAGAACCTCTTCTTCTTTTTTGACTGACGAC1500.10016426940181898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT2100.0151.666669
GCAGGGG950.0141.84219
GCAAAAG7500.0129.033333
CGAAAGC1650.0126.212124
GCTTATA302.4031733E-4122.500012
TATAGGC302.4031733E-4122.500017
AGGGTGT404.9160262E-6122.499996
AGCAAAA7950.0120.1886752
AAGCAGG10200.0114.093147
AGCAGGT4900.0105.000018
AGCTTAT354.437694E-4105.01
TAGGCAT354.437694E-4105.09
ACAAGGT706.1099854E-9105.08
GCGAAAG2100.099.1666643
CAAAAGC10200.096.078434
GAGCGAA2200.094.6590961
AGCAGGG5350.084.719638
TCGGGAG603.6827914E-581.666676
CTCGGGA603.6827914E-581.666675
AGCGAAA2650.078.584912
Produced by FastQC (version 0.11.2)