FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_114.3510000005a458.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_114.3510000005a458.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144860
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT11990.8276957061990888No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8200.5660637857241474No Hit
GTATGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC6210.4286897694325556No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5450.37622532099958583No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA5020.3465414883335634No Hit
TTTTTGGACAGTATGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4870.336186662984951No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4290.2961480049703162No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3500.24161259146762393No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3000.20709650697224904No Hit
ACTCTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATC2830.19536103824382162No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2810.19398039486400662No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2740.18914814303465416No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2680.18500621289520916No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA2660.1836255695153942No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2640.1822449261355792No Hit
ATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCAT2630.18155460444567167No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2580.1781029959961342No Hit
ACTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA2550.1760320309264117No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2200.15187077177964933No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2090.14427723319066685No Hit
ATATAAATCAGAAGCATGGCCCATTGGGGAGTCCCCCAAAGGAGTGGAAG2010.13875465967140688No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2000.1380643379814994No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1920.1325417644622394No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1910.1318514427723319No Hit
GGGTACACTATGGACACAGTCAACAGAACAAACACAAGCCAAAGGGGAAT1900.13116112108242442No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1900.13116112108242442No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1880.12978047770260942No Hit
GAACTCTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAA1860.12839983432279442No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1830.12632886925307193No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1800.12425790418334944No Hit
AGATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGA1750.12080629573381196No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1650.11390307883473699No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT1650.11390307883473699No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1630.112522435454922No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1610.111141792075107No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1600.1104514703851995No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1600.1104514703851995No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1590.10976114869529201No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1590.10976114869529201No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA1560.10769018362556952No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1530.10561921855584704No Hit
GAACAAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAG1530.10561921855584704No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA1530.10561921855584704No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1510.10423857517603202No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1490.10285793179621705No Hit
GAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGT1460.10078696672649455No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT1460.10078696672649455No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT1460.10078696672649455No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1450.10009664503658705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT900.0176.944449
GCAGGGG650.0169.615399
GCAAAAG8050.0124.782613
AAGCAGG9800.0123.757
GAGCGAA1800.0115.694451
CGAAAGC1850.0112.5675664
ACTTAAT901.8189894E-12108.888891
AGCAGGT6650.0106.84218
CGCTCCC354.4373982E-4105.06
GGTAGTT354.4373982E-4105.07
CAAAAGC9800.0102.54
AGCAGGG2700.099.814828
GGGTAGG407.5453846E-491.875011
GCTCCCC407.5453846E-491.875017
AGCAAAA11350.090.660792
GCAGGTA6500.090.461549
TCCAACT852.352499E-886.470592
AGCGAAA2450.085.02
GTAGGAG450.001204694681.666673
AAAGCAG14900.081.3926246
Produced by FastQC (version 0.11.2)