FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_112.3510000005a44b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_112.3510000005a44b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165445
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8190.49502855933996187No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT5570.3366677747892049No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4270.2580918129892109No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3690.2230348454169059No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2930.17709812928767868No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2710.16380065882921818No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2690.16259179787844902No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2650.16017407597691075No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2640.1595696455015262No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2510.1517120493215268No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2440.1474810359938348No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2180.131765843633836No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2170.13116141315845145No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2090.1263259693553749No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2030.1226993865030675No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2000.12088609507691378No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1940.11725951222460636No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1930.11665508174922179No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1890.11423735984768353No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA1890.11423735984768353No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1880.11363292937229895No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1820.11000634651999154No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1770.10698419414306869No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1770.10698419414306869No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1740.10517090271691498No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1710.10335761129076128No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1700.10275318081537671No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCAT1670.100939889389223No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGAAG100.0014750628244.999982
GCAGGGG1450.0177.41389
GACTTTA150.0049631414163.333331
GATATAG150.0049631414163.333332
GCAGGGT950.0141.84219
GCTTATA302.4036238E-4122.52
CGTACTC653.6525307E-9113.076913
TCGTACT653.6525307E-9113.076912
GTATTAA551.7856655E-7111.363636
AGCTTAT354.4385227E-4105.01
GTCGTAC706.1136234E-9105.01
TAGGCAT354.4385227E-4105.09
CAAAAGC8150.0100.705534
CGAAAGC1350.099.814823
AGCAAAA8000.099.531252
AAGCAGG9450.097.222227
TCTATGC654.8313996E-794.230773
GCTATAA552.3924471E-589.0909041
GCAAAAG9300.085.618283
AGCAGGG4650.084.301078
Produced by FastQC (version 0.11.2)