FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_107.3510000005a280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_107.3510000005a280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139437
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA12340.8849874853876661No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4700.33706978778946767No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4470.3205748832806214No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4150.29762545092048737No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTGTTTGGGTCTCCATTCCC4060.29117092306919967No Hit
GTACTGGAACTAGGATGAGTTCCAATGGCTCTCATTGCCTGCACCATCTG4010.2875850742629288No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3690.2646356419027948No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATC3590.25746394429025293No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG3540.253878095483982No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3130.22447413527256038No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC3100.2223226259887978No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG2790.200090363389918No Hit
CACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAAC2750.19722168434490125No Hit
TTCCAGTACAGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2730.1957873448223929No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGC2730.1957873448223929No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2660.1907671564936136No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2650.19004998673235943No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2610.18718130768734267No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2550.18287828911981754No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2550.18287828911981754No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2530.18144394959730917No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC2330.16710055437222543No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2320.16638338461097127No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2310.16566621484971705No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT2190.15706017771466685No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2180.15634300795341263No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2110.15132281962463334No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT2100.15060564986337915No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2080.14917131034087078No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2010.1441511220120915No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1930.13841376392205798No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1820.13052489654826194No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1790.12837338726449937No Hit
GGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT1680.12048451989070334No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA1600.11474716180066982No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1590.11402999203941565No Hit
CCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT1540.11044414323314472No Hit
CTCTTAGACCTGTACTGGAACTAGGATGAGTTCCAATGGCTCTCATTGCC1530.10972697347189055No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1520.10900980371063634No Hit
CAAGTTGTAGACCAGAAACTGAGTGATGCCCCATTCCTTGATCGGCTTCG1520.10900980371063634No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTAGGG1480.1061411246656196No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC1470.1054239549043654No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1430.10255527585934868No Hit
CAAAAACTTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA1420.10183810609809447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA301.1742522E-6163.33333245
GCAGGGT1100.0133.636379
GCAAAAG4800.0130.156233
CGAAAGC1250.0127.3999944
ACAAGGT404.914822E-6122.500017
AGGGTGT302.402821E-4122.499996
CCATAAC302.402821E-4122.499992
CAGGGTG551.784174E-7111.363648
CGTACTC354.4370434E-4105.03
TCGTACT354.4370434E-4105.02
AGGGTGA602.996785E-7102.083339
GCGAAAG1700.093.676473
GTACTGA407.544784E-491.875016
AAGCAGG8650.090.635837
GCAGGTA4850.088.402069
AGCAGGG3050.088.360668
CAAAAGC7700.082.727274
TAGAAAC1051.0859367E-981.6666642
GTAGAAA1051.0859367E-981.6666641
TAACTAT450.001204599281.6666645
Produced by FastQC (version 0.11.2)