FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_103.3510000005a209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_103.3510000005a209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123349
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8080.6550519258364478No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6910.5601991098428037No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3940.31941888462816886No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3390.2748299540328661No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTGTTTGGGTCTCCATTCCC3280.2659121679138055No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2730.22132323731850279No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2720.22051252948949726No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2560.20754120422540923No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2460.19943412593535415No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG2440.19781271027734312No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2330.1888949241582826No Hit
GTACTGGAACTAGGATGAGTTCCAATGGCTCTCATTGCCTGCACCATCTG2320.1880842163292771No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA2300.18646280067126608No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC2240.18159855369723304No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2170.17592359889419454No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2140.173491475407178No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2060.167005812775134No Hit
TTCCAGTACAGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2030.16457368928811744No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2030.16457368928811744No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG1940.1572773188270679No Hit
GTTAAAGCACAGATTTGAAATAATCGAAGGGAGAGACAGAACAATGGCCT1880.15241307185303488No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT1850.14998094836601836No Hit
CTAAAAATCTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGGGA1830.14835953270800736No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1760.14268457790496883No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1750.1418738700759633No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1690.1370096231019303No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1690.1370096231019303No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1580.12809183698286974No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1550.12565971349585323No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1550.12565971349585323No Hit
GATCCTTAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTCCGATG1510.12241688217983122No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA1510.12241688217983122No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1500.1216061743508257No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1500.1216061743508257No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1490.12079546652182019No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1450.11755263520579819No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1430.11593121954778718No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1410.11430980388977617No Hit
GATTTTTAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTCCGATG1390.11268838823176516No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1370.11106697257375414No Hit
CTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAGCTCTCAGTTA1370.11106697257375414No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1360.11025626474474863No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT1310.10620272559972112No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1290.10458130994171011No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA1280.10377060211270461No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA1280.10377060211270461No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1280.10377060211270461No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1270.1029598942836991No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAAAC251.16222574E-4147.03
GCAGGGT1000.0147.09
GCAAAAG5800.0135.172413
AGCAGGT5100.0134.509818
TCCAACT551.1405064E-9133.636372
CGAAAGC1200.0122.54
ACAAGGT501.0099393E-7122.58
CAAAAGC6600.0120.643944
AAGCAGG7850.0118.598747
GCAGGTA5000.0112.7000059
CAACTCT706.1008905E-9105.04
GCAGGTC1350.099.814829
AGGGTGT759.853466E-998.06
TCTATGC759.853466E-998.08
TAAACTA407.542699E-491.875015
AGCAAAA8950.090.335192
CTATGCT852.3495886E-886.470599
GAGCGAA1800.081.666671
GGTACAC603.6802063E-581.6666642
TATGGAC603.6802063E-581.6666649
Produced by FastQC (version 0.11.2)