FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_04b9251010.3510000005a58d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_04b9251010.3510000005a58d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48453
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC150.0049523762163.33333245
CGTGTTT150.0049523762163.333333
TACTGGT450.001199828881.666674
ATTATAG550.002660108366.8181841
AACCCGA600.0037552961.256
CAAAGAT750.00907933949.0000049
CGAGTAT150.0091230349.0230-234
GTATTTA1055.823516E-446.6666641
CTCTGGA1055.823516E-446.6666642
GGCCTGG657.839108E-626.38461735-39
TACAACA1050.00591740214.0230-234
CCATTCC8357.537516E-411.73652654
TCCATTC11250.0089408828.7111113
Produced by FastQC (version 0.11.2)