FastQCFastQC Report
Tue 28 Jun 2016
000000000-AP76Y_l01n01_04b9092010.3510000005a5a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP76Y_l01n01_04b9092010.3510000005a5a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10325
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAGG150.004896148163.333332
CTGGTGG150.004896148163.333331
ATTGTAG150.004896148163.333337
TGTAGTG150.004896148163.333339
CCCTGAC150.004896148163.333332
AATTGTA150.004896148163.333336
CTATAGT150.00908257149.060-64
CTAAATG150.00908257149.065-69
TCTTCAT150.00908257149.020-24
GTAGCTC150.00908257149.010-14
TTGCCAA150.00908257149.055-59
ATACAAC300.00336320632.666668225-229
TTTTTTT4804.4748675E-57.145833590-94