FastQCFastQC Report
Tue 21 Jun 2016
000000000-AP3VM_l01n03.33300000058c52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3VM_l01n03.33300000058c52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15629972
Sequences flagged as poor quality0
Sequence length251
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTAGTA301659119.3000409725622No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG4081972.6116297585178017No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATATAGCTTTGTG3298302.1102405045895156No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2985181.9099074521694601No Hit
CCTGTTTGATTCCCACGCTTTCGTGCTTCAGCGTCAGTGACAGTTTAGTA2856091.8273161333878267No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTATAGTC2315911.4817109077354713No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT2270131.452421028009519No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA2100471.3438731688067003No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGTT2027281.2970464694370534No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGTGTCAGTTAAGATCCAGGT1895881.2129772209444776No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTTTAGTGCCAGAT1654331.0584343977071744No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTATAGTGCCAGAT1617291.0347363386191606No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTGTACCCCAGTG1613261.032157959080157No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT1528440.9778904274428643No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1388990.888670817836398No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT1376070.8804046481977064No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGATTTAGTA1165050.7453948094084877No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTGAGCGTCAGTTATAGTTTAGTA1154330.7385361918754557No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA1145620.7329635651298672No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGAACCAGGT1134290.7257146717857205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1121020.7172245734029467No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGTGTCAGTTATAGTTTAGTG1043450.6675955657502137No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTTTAGTA994720.6364182866098544No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG973010.6225283065126412No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTAGCTTAGTA937970.6001098402479543No Hit
CCTGTTTGATACCCACACTTTCGTGCTTCAGTGTCAGTTGTGGCCTGGTA904880.5789389769860112No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATCCAGCTTAGAA898120.5746139532431663No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTCAGCGTCAGTTTCTGCCCAGAT876200.5605896158995038No Hit
CCTGTTTGCTACCCACGCTTTCGCATCTGAGCGTCAGTCATCGTCCAGAA870240.5567764292859898No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCCGAAATAGATTTGTC818530.5236925568388734No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTTGTCACCCAGCA760650.4866611405317936No Hit
CCTGTTTGCTCCCCATACTTTCGAGCCTCAGCGTCAGTAAATGCCCAGTA723200.4627007649149979No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTGTGCCAGGT696260.44546464958478493No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATAGACTTAGTA680310.4352598968187531No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATGTGGCTTTGTG675870.43241920075096746No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGT663890.4247544397392395No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTACACTCCAGTG628060.4018305343093385No Hit
CCTGTTTGATCCCCACGCTTTCGCATCTCAGCGTCAGTACTGCACCAGGT614630.39323806850069853No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT548410.3508707501203457No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTAGATTAGTA521480.33364103275424933No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA520140.3327837055626203No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAATATTGGTCCAGAA507230.32452393388804535No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGTCTAGAA504480.32276449375597094No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAATGTCCAGCA497610.31836909240784306No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGAATAAGCTTTGTG487320.3117855873318263No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATGGTCCAGCC461690.2953876053008924No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT445890.2852788219966101No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTCTTGGTCCAGAT443900.28400562713739985No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTGACATTCTAGCA441040.28217580940004244No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTCCGGATTAGCC428160.27393523161781735No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTG420670.26914315649445825No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGGATAGTC415970.26613611335964005No Hit
CCTGTTTGATCCCCACGCTTTCGCATCTCAGCGTCAGTACTGCACCAGAT412110.2636664992106192No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGTA388040.24826659958188027No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAT387720.24806186473014794No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATCAGGCTTTGTG381280.24394157583903542No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTCCAGTT377410.2414655637258979No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA354500.22680782793468857No Hit
CCTGTTTGATACCCACGCTTTCGTGCCTCAGCGTCAGTTATACTATAGTA354380.22673105236528895No Hit
CCTGTTTGCTCCCCATACTTTCGAGCCTCAGCGTCAGTTAATGTCCAGCA353320.2260528681689257No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG352670.22563700050134447No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGTATAGTA347700.22245721233537719No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA347370.22224607951952824No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTTTTGTGCCAGAT345260.2208961090909184No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA332220.2125531638828272No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATGGTCCAGAA319170.20420382071061932No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACGTCCCAGCA306750.1962575492777594No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATCAGCGTCAGTTAAGGCCCAGTA290950.18614876597347713No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTGAGCGTCAGTTGTAAGTTTGTA285910.1829241920586934No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGCA280190.17926455658397852No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATATGGCTTTGTG275520.17627670734151027No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGCA273150.17476038984586795No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAACGTCAGTAATAGCCCAGCA269790.17261067390267879No Hit
CCCTTTCGCTACCCTGGCCTTCGTACCTCAGCGTCAGTTAATGTCCAGGA256840.16432531037163725No Hit
CCTGTTTGCTCCCCATACTTTCGAGCCTCAGCGTCAGTTGATGCCCAGTA256620.1641845551610713No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATAATGAATTAGTC250730.16041615429637368No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATTTAGCTTTGTC249920.15989791920292626No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATAATGAATTAGCC242140.15492030312018473No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTACATCCCAGAT240650.1539670064668062No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA233700.1495204214057453No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTAGTAAGTTTGTA231610.14818324690536874No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGGATAGTC231110.14786334869953702No Hit
CCCGTTCGCTACCCTGGCTTTCGCGCCTGAGCGTCAGACACAGTCCAGAA222780.14253384459038057No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCAACGTAGATTGGTA222580.1424058853080479No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTGTTAGGCCAGAT215970.13817683102695258No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGGT198460.1269739958587258No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGTAGCTTAGTA197490.12635339333941226No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTGATAGCTTAGTA190880.12212433905831692No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGTA181350.11602707925516438No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAATGCCCAGCA179070.11456834343657174No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACGGGCCAGAT175720.11242502545749922No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATGGCCTAGAA175600.1123482498880996No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGACTTAGTA170540.10911088004508263No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATCTAGTATTAGCC170220.1089061451933503No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGAAATAACCCAGCA169990.10875899201866772No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAACATCCTAGTG169570.10849027752576908No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTGAGCGTCAGTTACAGTTTAGTA165680.10600146948439831No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGTGCCAGGT163050.10431880492172346No Hit
CCTGTTCGCTACCCACGCTTTCGTGCCTGAGCGTCAGATGTAGATTAGAG160770.10286006910313084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC9302850.0244.885443
TGTTTGA4057300.0244.731263
TTTGCTC9005800.0244.668085
TTGATAC2760700.0244.538536
CTGTTTG13379450.0244.170472
GTTTGCT9342300.0244.120164
CTGTTCG344050.0243.326552
CTCCCCA9278100.0242.750189
TTGCTCC9196850.0241.749986
CCTGTTT13535850.0241.083131
TTTGATA2776450.0240.834965
TGATACC2800200.0240.406597
GCTCCCC9548650.0240.225028
GATACCC2815400.0239.67438
CCTGTTC350400.0239.511281
TGATCCC1018450.0238.1567
GTTTGAT4184550.0237.716524
CCTATTT299650.0237.4371
TGCTCCC9380100.0237.037587
CCCTCCG2175750.0236.56027245
Produced by FastQC (version 0.11.2)