FastQCFastQC Report
Tue 21 Jun 2016
000000000-AP3VM_l01n02.33200000058c55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3VM_l01n02.33200000058c55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15629972
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCGTATGCG6694344.283014710455015No Hit
CTCGATTAGATC4421582.8289110178828216No Hit
CACTCAACAGAC4350562.7834726767264844No Hit
GAGTCGCGTTTA4320612.764310774197164No Hit
AGTCACATCACT4284262.7410541746331982No Hit
CAATCTGTTGGT4018652.5711178497312726No Hit
ATCATCTGGGTT3999252.558705799345002No Hit
CATGACCTGGAG3864512.4724996308374703No Hit
ATCGCCACGTAA3775982.4158584545129065No Hit
CGTCGTCAAATG3730872.3869972383827687No Hit
TAGTACCCGAGG3711342.3745020144629816No Hit
CGGAGAAGGGAA3621802.3172146437626377No Hit
GCTATGGACCGA3578182.2893067242858787No Hit
CGGGCATAAGTC3517342.250381510600275No Hit
AGAGTAGCTAAG3516932.250119194071493No Hit
TGCCATCTGAAT3509672.2454742721228165No Hit
CAGTTGGTCTGA3496532.237067347273559No Hit
ATTACGGCGGCA3458272.2125887365633155No Hit
CTCGCTTCACTT3447312.2055765678914843No Hit
TCTTAGACGACG3244812.0760177945296383No Hit
GGACAACGCTTG3168542.027220522212068No Hit
GATACAGGTGAA3155442.018839189219277No Hit
TTATCCGAGGTT3095361.9804002208065374No Hit
ATTCAGCACCAG3070091.9642325654838027No Hit
ACCAAGGGCACT3048881.950662483592421No Hit
CTACACAAGCAC3014241.9284999358923995No Hit
GTAGTAGGCCAC2865771.8335093626527288No Hit
TGCACCTCTGTC2763071.767802271174894No Hit
GACTAACGTCAC2704571.7303741810925828No Hit
TTACCAAGACTC2701971.7287107104222579No Hit
CGAGTCTAGTTG2676411.7123575141401406No Hit
GTTTGGCGACGA2662781.7036370890491679No Hit
GGACCGAACTCA2615501.6733875147057204No Hit
CGACAGCTGACA2548711.6306555123707194No Hit
GTACGAATCCTG2545221.628422622894014No Hit
GGCATGCATGTT2395001.5323124059339326No Hit
TCAGTACGAGGC2394281.531851752517535No Hit
TCACGTCGTTCC2301481.472478645515168No Hit
CGTTTCCGTTTC1978681.2659523638302104No Hit
CAAGACACGACC627560.40151063610350674No Hit
GGAATAAACGTG550030.3519072203072405No Hit
ACACGGTGTCTA510290.3264817109077355No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG1900.06.00000056
TACGGAG953.4298391E-66.00000053
GCCTGTT953.4298391E-66.00000056
ACAGGTA953.4298391E-66.00000054
GGCGGCC7150.06.00000056
TTACGGG953.4298391E-66.00000052
CGGAGCA1900.06.00000051
CTGGACC1900.06.00000054
CGGACAT1156.827213E-86.01
CTAGATG600.0033965676.06
GGTCGTG600.0033965676.02
CGAAATC600.0033965676.05
GCGGTTA1259.677024E-96.06
CGCGGAT650.00126197076.06
TGGACTG1156.827213E-86.05
ACCGCGG1101.8153514E-76.04
GTCCTGG600.0033965676.04
TTTCGCG1507.4578566E-116.02
CAGTTAG852.4449742E-56.01
GCTATTG600.0033965676.01
Produced by FastQC (version 0.11.2)